Abstract

The recently established Automated DNA Sequencing Facility in the McArdle Laboratory for Cancer Research provides scientists access to state-of-the- art machines for rapid DNA sequencing. All five projects of this program- project grant currently make heavy use of this common facility. The projected needs for this facility will increase in future years. Uses include sequence verification of recombinant DNAs, and screening of candidate cDNA from screening projects such as yeast two-hybrid and protein sequencing. Additionally, all faculty of this PPG are using rodent liver as a model of carcinogenesis. Therefore, an expressed sequence tag data base will be established on this important tissue. Funds are requested to support the costs for running the facility proportional to its use by the projects supported by this program-project grant. Only costs incurred for running samples on the common machines and the maintenance of those machines are requested under this core. All other costs for the preparation of sequencing samples are borne by individual projects. This core will be administered by Dr. Bradfielde.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA022484-23
Application #
6300178
Study Section
Project Start
2000-02-11
Project End
2001-01-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
23
Fiscal Year
2000
Total Cost
$239,758
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Xia, Chuanwu; Rwere, Freeborn; Im, Sangchoul et al. (2018) Structural and Kinetic Studies of Asp632 Mutants and Fully Reduced NADPH-Cytochrome P450 Oxidoreductase Define the Role of Asp632 Loop Dynamics in the Control of NADPH Binding and Hydride Transfer. Biochemistry 57:945-962
Oberley, Christopher C; Bilger, Andrea; Drinkwater, Norman R (2015) Genetic background determines if Stat5b suppresses or enhances murine hepatocarcinogenesis. Mol Carcinog 54:959-70
Kennedy, Gregory D; Nukaya, Manabu; Moran, Susan M et al. (2014) Liver tumor promotion by 2,3,7,8-tetrachlorodibenzo-p-dioxin is dependent on the aryl hydrocarbon receptor and TNF/IL-1 receptors. Toxicol Sci 140:135-43
Copp, Richard R; Peebles, Daniel D; Soref, Cheryl M et al. (2013) Radioprotective efficacy and toxicity of a new family of aminothiol analogs. Int J Radiat Biol 89:485-92
Figueiredo, Marxa L; Stein, Timothy J; Jochem, Adam et al. (2012) Mutant Hras(G12V) and Kras(G12D) have overlapping, but non-identical effects on hepatocyte growth and transformation frequency in transgenic mice. Liver Int 32:582-91
Stein, Timothy J; Jochem, Adam; Holmes, Katie E et al. (2011) Effect of mutant ?-catenin on liver growth homeostasis and hepatocarcinogenesis in transgenic mice. Liver Int 31:303-12
Copp, Richard R; Peebles, Daniel D; Fahl, William E (2011) Synthesis and growth regulatory activity of a prototype member of a new family of aminothiol radioprotectors. Bioorg Med Chem Lett 21:7426-30
Stein, Timothy J; Bowden, Margaret; Sandgren, Eric P (2011) Minimal cooperation between mutant Hras and c-myc or TGF? in the regulation of mouse hepatocyte growth or transformation in vivo. Liver Int 31:1298-305
Xia, Chuanwu; Hamdane, Djemel; Shen, Anna L et al. (2011) Conformational changes of NADPH-cytochrome P450 oxidoreductase are essential for catalysis and cofactor binding. J Biol Chem 286:16246-60
Figueiredo, Marxa L; Wentworth, Kristin M; Sandgren, Eric P (2010) Quantifying growth and transformation frequency of oncogene-expressing mouse hepatocytes in vivo. Hepatology 52:634-43

Showing the most recent 10 out of 258 publications