Abstract

This grant addresses the role of autologous bone marrow transplantation in acute leukemia, myeloma and diffuse large cell lymphoma when combined with high-dose chemotherapy and total body irradiation. As these are bone marrow based diseases, the question of purging to remove malignant cells before transplantation is evident. Two new separation procedures have been developed, in vitro immunocolloid separation and pharmacological tumor cell reduction. We propose to study the effect of the separation technique on hematopoietic reconstruction capacity and the efficacy of each separation as measured by time to tumor recurrence. Detection systems for leukemic cells have been developed using molecular probes and will be applied for monitoring bone marrow for leukemic cell contamination according to method of purging. Well defined tumor markers will be employed in transplants in myeloma.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA023077-07
Application #
3093021
Study Section
Clinical Cancer Program Project Review Committee (CCP)
Project Start
1979-01-01
Project End
1990-07-31
Budget Start
1987-08-01
Budget End
1988-07-31
Support Year
7
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of Texas MD Anderson Cancer Center
Department
Type
Hospitals
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Takaue, Y; Watanabe, T; Kawano, Y et al. (1991) A simplified and rapid limiting dilution assay of T lymphocytes. Cell Immunol 133:526-31
Barlogie, B; Gahrton, G (1991) Bone marrow transplantation in multiple myeloma. Bone Marrow Transplant 7:71-9
Yau, J C; Reading, C L; Thomas, M W et al. (1990) Purging of T-lymphocytes with magnetic affinity colloid. Exp Hematol 18:219-22
Takaue, Y; Kawano, Y; Reading, C L et al. (1990) Effects of recombinant human G-CSF, GM-CSF, IL-3, and IL-1 alpha on the growth of purified human peripheral blood progenitors. Blood 76:330-5
Dunphy, F R; Spitzer, G; Buzdar, A U et al. (1990) Treatment of estrogen receptor-negative or hormonally refractory breast cancer with double high-dose chemotherapy intensification and bone marrow support. J Clin Oncol 8:1207-16
Jagannath, S; Barlogie, B; Dicke, K et al. (1990) Autologous bone marrow transplantation in multiple myeloma: identification of prognostic factors. Blood 76:1860-6
Barlogie, B; Epstein, J (1990) Multiple myeloma: biology and therapy. J Cancer Res Clin Oncol 116:109-11
Horwitz, L J; Kantarjian, H M; Jagannath, S et al. (1989) Piperazinedione plus total body irradiation: an alternative preparative regimen for allogeneic bone marrow transplantation in advanced phases of chronic myelogenous leukemia. Bone Marrow Transplant 4:101-5
Bator, J M; Reading, C L (1989) Measurement of antibody affinity for cell surface antigens using an enzyme-linked immunosorbent assay. J Immunol Methods 125:167-76
Takaue, Y; Reading, C L; Watanabe, T et al. (1989) Cell-mediated suppression of human hematopoiesis: evaluation by limiting-dilution analysis of hematopoietic progenitors. Am J Hematol 32:205-11

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