Abstract

The goal of the proposed projects is to obtain an understanding of the fundamental aspects of carcinogenesis by studying interactions with cells of viruses and virus-related genes important to malignant transformation. Six interrelated but distinct systems will be analyzed to achieve this goal. Dr. J. Coffin will study the inheritance and expression of endogenous retroviruses important in murine leukemia. Dr. R. Schwartz will study the interaction of murine viruses with cell surface proteins and with the immune system. Dr. N. Rosenberg will study molecular events of in vitro transformation of lymphocytes by """"""""nontransforming"""""""" virus. Dr. T. Krontiris will study differential expression of virus-like oncogenes in lymphocytes. Dr. E. Androphy will study regulation of expression of genes important in papilloma virus transformation. Finally, Dr. B. Schaffhausen will study function of papovirus T-antigen important for transformation. It is anticipated that joint analysis of these distinct but intellectually interrelated systems will provide novel insights into the natural history of cancer induction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA024530-12
Application #
3093089
Study Section
Cancer Special Program Advisory Committee (CAK)
Project Start
1988-03-01
Project End
1992-12-31
Budget Start
1991-01-01
Budget End
1991-12-31
Support Year
12
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Tufts University
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02111

  Publications

Howes, S H; Bockus, B J; Schaffhausen, B S (1996) Genetic analysis of polyomavirus large T nuclear localization: nuclear localization is required for productive association with pRb family members. J Virol 70:3581-8
Corina, K; Grossman, S R; Barsoum, J et al. (1993) The tryptophan bridge is a critical feature of the papillomavirus E2 DNA binding domain. Virology 197:391-6
Prakash, S S; Grossman, S R; Pepinsky, R B et al. (1992) Amino acids necessary for DNA contact and dimerization imply novel motifs in the papillomavirus E2 trans-activator. Genes Dev 6:105-16
Runnels, J; Serunian, L; Thursby, M et al. (1991) Moloney leukemia virus immortalizes B lymphocytes in vitro. J Virol 65:5549-52
Morrissey, L C; Barsoum, J; Androphy, E J (1989) Trans activation by the bovine papillomavirus E2 protein in Saccharomyces cerevisiae. J Virol 63:4422-5
Zouali, M; Stollar, B D; Schwartz, R S (1988) Origin and diversification of anti-DNA antibodies. Immunol Rev 105:137-59
Schwartz, R S (1988) Polyvalent anti-DNA autoantibodies: immunochemical and biological significance. Int Rev Immunol 3:97-115
Sabbaga, J; Migliorini, P; Schwartz, R S (1988) Autoantibodies to DNA. In Vivo 2:61-5
Hubbert, N L; Schiller, J T; Lowy, D R et al. (1988) Bovine papilloma virus-transformed cells contain multiple E2 proteins. Proc Natl Acad Sci U S A 85:5864-8
Stoye, J P; Coffin, J M (1988) Polymorphism of murine endogenous proviruses revealed by using virus class-specific oligonucleotide probes. J Virol 62:168-75

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