While the mutational origin of cancer has increasingly been recognized, the ability to effectively monitor human populations for increased frequency of mutations has been an elusive goal. Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) has provided the means to analyze a large number of polypeptide gene products simultaneously. Whereas in previous years of funding for this project, the major emphasis was on development of the 2-D PAGE approach for the study of mutation, in this competing renewal application, the major aim is the actual detection of human germinal as well as somatic mutations following exposure to mutagens. We propose to determine the frequency of germinal mutations in cancer patients who have been treated with radiation and chemotherapy and in a control group. The frequency of in vivo somatic mutations will be determined in a group of patients with Hodgkin's disease receiving chemotherapy, before and after therapy, to determine the mutagenic impact of the treatment. In vitro somatic mutation studies will be expanded to include radiation as well as chemical mutagens. For the in vitro studies, the end points to be measured will be facilitated detected in 2-D gels. Scoring of two-dimensional gels will be facilitated by automated image analysis algorithms previously developed and which will undergo further refinement during the funding period.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA026803-08A1
Application #
3093156
Study Section
(SRC)
Project Start
1980-09-01
Project End
1993-03-31
Budget Start
1988-04-06
Budget End
1989-03-31
Support Year
8
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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