Serum Collection, Processing and Archiving. This Core has been collecting, processing andinventorying serum samples from patients who have been participating in various research protocols of thisgrant including MSLT-I and MSLT-II. During the period from May 1, 2007 to February 29, 2008, a total of1,439 blood/serum samples (239 samples from MSLT-I, 739 samples from MSLT-II and 461 samples fromother protocols) were received, processed and catalogued in the database. The processing of the specimensincluded separation of serum from clotted blood and aliquoted into 1.0ml portion under sterile and bio-safeenvironment. Special attention was placed on accuracy of information on label of each vial and correspondinginformation that was entered into the database. During this reporting period (May 1, 2006 to February 29,2008), a total of 7,302 vials were prepared and archived in -35 C freezers for later use. Each vial's label wasgiven a code to maintain subject's confidentiality. The personnel responsible for archiving the serum sampleswere also responsible for triaging the serum samples to various immunologic testing labs, and for retrieval atthe request of various investigators and after approval by the PI of this grant.Other specimens were received for Molecular Biology laboratory's use such as frozen sera, blood forRT-PCR, and FTA cards. A total of 800 specimens (417 tubes of blood, 2,281 vials of frozen sera, and 263FTA cards) were received for Molecular Biology laboratory's use.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA029605-27
Application #
7728764
Study Section
Subcommittee G - Education (NCI)
Project Start
2008-06-01
Project End
2009-05-31
Budget Start
2008-06-01
Budget End
2009-05-31
Support Year
27
Fiscal Year
2008
Total Cost
$159,158
Indirect Cost
Name
John Wayne Cancer Institute
Department
Type
DUNS #
556074458
City
Santa Monica
State
CA
Country
United States
Zip Code
90404
Jones, Maris S; Lee, Jihey; Stern, Stacey L et al. (2017) Is Pregnancy-Associated Melanoma Associated with Adverse Outcomes? J Am Coll Surg 225:149-158
Faries, Mark B; Thompson, John F; Cochran, Alistair J et al. (2017) Completion Dissection or Observation for Sentinel-Node Metastasis in Melanoma. N Engl J Med 376:2211-2222
Ozao-Choy, Junko; Nelson, Daniel W; Hiles, Jason et al. (2017) The prognostic importance of scalp location in primary head and neck melanoma. J Surg Oncol 116:337-343
Karakousis, Giorgos; Gimotty, Phyllis A; Bartlett, Edmund K et al. (2017) Thin Melanoma with Nodal Involvement: Analysis of Demographic, Pathologic, and Treatment Factors with Regard to Prognosis. Ann Surg Oncol 24:952-959
Jones, Maris S; Torisu-Itakura, Hitoe; Flaherty, Devin C et al. (2016) Second Primary Melanoma: Risk Factors, Histopathologic Features, Survival, and Implications for Follow-Up. Am Surg 82:1009-1013
Faries, Mark B (2016) Intralesional Immunotherapy for Metastatic Melanoma: The Oldest and Newest Treatment in Oncology. Crit Rev Oncog 21:65-73
Ono, Shigeshi; Oyama, Takashi; Lam, Stella et al. (2015) A direct plasma assay of circulating microRNA-210 of hypoxia can identify early systemic metastasis recurrence in melanoma patients. Oncotarget 6:7053-64
Wang, Jinhua; Huang, Sharon K; Marzese, Diego M et al. (2015) Epigenetic changes of EGFR have an important role in BRAF inhibitor-resistant cutaneous melanomas. J Invest Dermatol 135:532-541
Cochran, Alistair J; Huang, Rong-Rong; Su, Albert et al. (2015) Is sentinel node susceptibility to metastases related to nodal immune modulation? Cancer J 21:39-46
Marzese, Diego M; Huang, Sharon K; Hoon, Dave S B (2015) In Situ Sodium Bisulfite Modification of Genomic DNA from Microdissected Melanoma Paraffin-Embedded Archival Tissues. Methods Mol Biol :

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