Abstract

This core will provide centralized services to the individual research projects in this Program Project and will also provide reagents needed by several investigators. The core will provide well-characterized stocks of cultured mouse keratinocytes, and provide expertise for subcloning (nested deletions) and template purification for DNA sequencing. A plasmid bank will be maintained to allow all investigators access to appropriate expression vectors and DNA probes, TGF-beta and EGF will be purchased in bulk quantities and stored by the core facility. The core will also prepare tissue culture media, bacterial culture media, agar plates, and sterilize and bake glassware.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA048799-03
Application #
3808010
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212

  Publications

Satterwhite, D J; White, R L; Aakre, M E et al. (2001) TGF-beta1 regulates the expression of multiple max-interacting transcription factors in Balb/MK cells: implications for understanding the mechanism of action of TGF-beta1. Pediatr Res 50:67-75
Engel, M E; McDonnell, M A; Law, B K et al. (1999) Interdependent SMAD and JNK signaling in transforming growth factor-beta-mediated transcription. J Biol Chem 274:37413-20
Norgaard, P; Law, B; Joseph, H et al. (1999) Treatment with farnesyl-protein transferase inhibitor induces regression of mammary tumors in transforming growth factor (TGF) alpha and TGF alpha/neu transgenic mice by inhibition of mitogenic activity and induction of apoptosis. Clin Cancer Res 5:35-42
Lutterbach, B; Hann, S R (1999) c-Myc transactivation domain-associated kinases: questionable role for map kinases in c-Myc phosphorylation. J Cell Biochem 72:483-91
Gorska, A E; Joseph, H; Derynck, R et al. (1998) Dominant-negative interference of the transforming growth factor beta type II receptor in mammary gland epithelium results in alveolar hyperplasia and differentiation in virgin mice. Cell Growth Differ 9:229-38
Alexandrow, M G; Moses, H L (1998) c-myc-enhanced S phase entry in keratinocytes is associated with positive and negative effects on cyclin-dependent kinases. J Cell Biochem 70:528-42
Zhao, G Q; Liaw, L; Hogan, B L (1998) Bone morphogenetic protein 8A plays a role in the maintenance of spermatogenesis and the integrity of the epididymis. Development 125:1103-12
Engel, M E; Datta, P K; Moses, H L (1998) RhoB is stabilized by transforming growth factor beta and antagonizes transcriptional activation. J Biol Chem 273:9921-6
Datta, P K; Chytil, A; Gorska, A E et al. (1998) Identification of STRAP, a novel WD domain protein in transforming growth factor-beta signaling. J Biol Chem 273:34671-4
Serra, R; Johnson, M; Filvaroff, E H et al. (1997) Expression of a truncated, kinase-defective TGF-beta type II receptor in mouse skeletal tissue promotes terminal chondrocyte differentiation and osteoarthritis. J Cell Biol 139:541-52

Showing the most recent 10 out of 62 publications