Abstract

The Core Facility for this Program Project will store, supply and run quality control on the heterocyclic amine chemicals. The chemicals will be available from either stocks on hand, commercial sources, or will be synthesized as needed, here at LLNL or at the Swedish University of Agricultural Sciences. Radiolabeled compounds will also be supplied from current stocks or synthesized. Top priority is 3H labeled PHlP and PhlP metabolites. The core will provide mass spectrometry analysis of metagenic heterocyclic amines from foods and mutagen/carcinogen-forming model reactions, metabolites of the heterocyclic amines, some DNA- and protein-adducts from in vivo and in vitro experiments associated with the Metabolism and Dosimetry projects. The core will also provide gas chromatography-mass spectrometry analysis of food extracts for the Chemistry Project. Foods and various experimental samples will be tested using the Ames/Salmonella test. Mutagenic testing will be done prior to chemical analysis. Synthetic products, metabolites and mutagen-forming reactions will also be tested for mutagenic activity in support of a number of the Projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA055861-03
Application #
3751603
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Lawrence Livermore National Laboratory
Department
Type
DUNS #
827171463
City
Livermore
State
CA
Country
United States
Zip Code
94550

  Publications

Turesky, Robert J; Goodenough, Angela K; Ni, Weijuan et al. (2007) Identification of 2-amino-1,7-dimethylimidazo[4,5-g]quinoxaline: an abundant mutagenic heterocyclic aromatic amine formed in cooked beef. Chem Res Toxicol 20:520-30
Bogen, K T; Keating 2nd, G A; Chan, J M et al. (2007) Highly elevated PSA and dietary PhIP intake in a prospective clinic-based study among African Americans. Prostate Cancer Prostatic Dis 10:261-9
Keating, Garrett A; Bogen, Kenneth T; Chan, June M (2007) Development of a meat frequency questionnaire for use in diet and cancer studies. J Am Diet Assoc 107:1356-62
Keating, G A; Bogen, K T (2004) Estimates of heterocyclic amine intake in the US population. J Chromatogr B Analyt Technol Biomed Life Sci 802:127-33
Wogan, Gerald N; Hecht, Stephen S; Felton, James S et al. (2004) Environmental and chemical carcinogenesis. Semin Cancer Biol 14:473-86
Keating, G A; Bogen, K T (2001) Methods for estimating heterocyclic amine concentrations in cooked meats in the US diet. Food Chem Toxicol 39:29-43
Hatch, F T; Knize, M G; Colvin, M E (2001) Extended quantitative structure-activity relationships for 80 aromatic and heterocyclic amines: structural, electronic, and hydropathic factors affecting mutagenic potency. Environ Mol Mutagen 38:268-91
Bogen, K T; Enns, L; Hall, L C et al. (2001) Gel microdrop flow cytometry assay for low-dose studies of chemical and radiation cytotoxicity. Toxicology 160:5-10
Hatch, F T; Lightstone, F C; Colvin, M E (2000) Quantitative structure-activity relationship of flavonoids for inhibition of heterocyclic amine mutagenicity. Environ Mol Mutagen 35:279-99
Matsumoto, K; Tucker, J D (1998) Detection of structural chromosome damage in rat interphase cells using region-specific fluorescence in situ hybridization probes developed by microdissection. Mutat Res 421:179-90

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