Abstract

The SCID mouse colony core facility serves this program project by providing a biologic model to test the tumorigenicity of prostate carcinoma in vivo. Specifically, by use of the SCID mouse peritoneal model we may test tumor growth, adhesiveness, penetration, local spread and distant metastasis. The specific measurable end points are both qualitative and quantitative. Qualitative assessments include gross anatomic as well as histologic and phenotypic descriptions of the immunotopography of human prostate xenografts. Quantitative assessment includes tumor xenograft size and number, and animal survival data. Specifically the SCID model will be used to: 1) Assess in vivo spread and metastasis of prostate cell lines selected or transfected for variable plasma membrane integrin status. 2) Assess in vivo spread and metastases of metalloproteinase and TIMP transfectants of a human prostate carcinoma cell line. 3) Provide fixed and frozen tissues from the SCID mouse tumors for analysis as described in Projects 1-3.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
1P01CA056666-01A1
Application #
3773920
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
University of Arizona
Department
Type
DUNS #
City
Tucson
State
AZ
Country
United States
Zip Code
85721

  Publications

Nagle, Raymond B; Algotar, Amit M; Cortez, Connie C et al. (2013) ERG overexpression and PTEN status predict capsular penetration in prostate carcinoma. Prostate 73:1233-40
Sroka, Isis C; Sandoval, Cynthia P; Chopra, Harsharon et al. (2011) Macrophage-dependent cleavage of the laminin receptor ?6?1 in prostate cancer. Mol Cancer Res 9:1319-28
Sroka, Isis C; Anderson, Todd A; McDaniel, Kathy M et al. (2010) The laminin binding integrin alpha6beta1 in prostate cancer perineural invasion. J Cell Physiol 224:283-8
Sroka, Isis C; Pond, Gerald D; Nagle, Raymond B et al. (2009) Human Cell Surface Receptors as Molecular Imaging Candidates for Metastatic Prostate Cancer. Open Prost Cancer J 2:59-66
Ports, Michael O; Nagle, Ray B; Pond, Gerald D et al. (2009) Extracellular engagement of alpha6 integrin inhibited urokinase-type plasminogen activator-mediated cleavage and delayed human prostate bone metastasis. Cancer Res 69:5007-14
King, Tamara E; Pawar, Sangita C; Majuta, Lisa et al. (2008) The role of alpha 6 integrin in prostate cancer migration and bone pain in a novel xenograft model. PLoS One 3:e3535
Demetriou, Manolis C; Stylianou, Panayiota; Andreou, Maria et al. (2008) Spatially and temporally regulated alpha6 integrin cleavage during Xenopus laevis development. Biochem Biophys Res Commun 366:779-85
Sroka, Isis C; McDaniel, Kathy; Nagle, Raymond B et al. (2008) Differential localization of MT1-MMP in human prostate cancer tissue: role of IGF-1R in MT1-MMP expression. Prostate 68:463-76
Demetriou, Manolis C; Kwei, Kevin A; Powell, Marianne B et al. (2008) Integrin A6 Cleavage in Mouse Skin Tumors. Open Cancer J 2:1-4
Sroka, Isis C; Chen, Man Ling; Cress, Anne E (2008) Simplified purification procedure of laminin-332 and laminin-511 from human cell lines. Biochem Biophys Res Commun 375:410-3

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