CORE COMPONENT B: Microscopy and Morphometrics The microscopy core facility has been reorganized under the leadership of a cell biologist and a pathologist to ensure that coverage of the diverse microscopy needs of the three projects are adequately maintained throughout the four years of this grant renewal and to direct the duties of the microscopist hired for this project. Microscopic techniques will be used to: 1) Evaluate the integrity of the diaphragm of SCID mice injected with the various cell lines (Project 2) using scanning electron microscopy; 2) Determine basement membrane invasion of SCID mouse organs and tissue by the different prostate cell lines using transmission electron microscopy; 3) Localize the expression of specific integrins, proteases, and extracellular matrix elements during tissue invasion in SCID mouse using confocal laser microscopy and immunogold probes at the TEM level (Projects 1, 2, & 3); 4) Perform confocal microscopy localization of matrilysin expression in tumors generated by subcutaneous and orthotopic injections in athymic nude mice by co-injection of LNCaP and prostate fibroblast cells (Project 3); 5) Perform video microscopy linear migration assay to evaluate the effects of substrate (laminin 5) on tumor cell migration (Project 1).

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Arizona
United States
Zip Code
Nagle, Raymond B; Algotar, Amit M; Cortez, Connie C et al. (2013) ERG overexpression and PTEN status predict capsular penetration in prostate carcinoma. Prostate 73:1233-40
Sroka, Isis C; Sandoval, Cynthia P; Chopra, Harsharon et al. (2011) Macrophage-dependent cleavage of the laminin receptor ?6?1 in prostate cancer. Mol Cancer Res 9:1319-28
Sroka, Isis C; Anderson, Todd A; McDaniel, Kathy M et al. (2010) The laminin binding integrin alpha6beta1 in prostate cancer perineural invasion. J Cell Physiol 224:283-8
Sroka, Isis C; Pond, Gerald D; Nagle, Raymond B et al. (2009) Human Cell Surface Receptors as Molecular Imaging Candidates for Metastatic Prostate Cancer. Open Prost Cancer J 2:59-66
Ports, Michael O; Nagle, Ray B; Pond, Gerald D et al. (2009) Extracellular engagement of alpha6 integrin inhibited urokinase-type plasminogen activator-mediated cleavage and delayed human prostate bone metastasis. Cancer Res 69:5007-14
Sroka, Isis C; McDaniel, Kathy; Nagle, Raymond B et al. (2008) Differential localization of MT1-MMP in human prostate cancer tissue: role of IGF-1R in MT1-MMP expression. Prostate 68:463-76
Demetriou, Manolis C; Kwei, Kevin A; Powell, Marianne B et al. (2008) Integrin A6 Cleavage in Mouse Skin Tumors. Open Cancer J 2:1-4
Sroka, Isis C; Chen, Man Ling; Cress, Anne E (2008) Simplified purification procedure of laminin-332 and laminin-511 from human cell lines. Biochem Biophys Res Commun 375:410-3
Moran, Carlos M; Garriock, Robert J; Miller, Melanie K et al. (2008) Expression of the fast twitch troponin complex, fTnT, fTnI and fTnC, in vascular smooth muscle. Cell Motil Cytoskeleton 65:652-61
King, Tamara E; Pawar, Sangita C; Majuta, Lisa et al. (2008) The role of alpha 6 integrin in prostate cancer migration and bone pain in a novel xenograft model. PLoS One 3:e3535

Showing the most recent 10 out of 68 publications