This core will develop highly selective, high affinity antibodies to investigate coordinated cell signaling.Antibodies will be generated to distinguish active forms of target enzymes. The antibodies will be isolatedfrom an established cDNA, single-chain immune library displayed on ribosomes. This library will bescreened using modified peptides that mimic the effector regions of key signal proteins or, alternatively, fulllengthactivated proteins. This objective will be fulfilled in two specific aims:1. To maintain immune libraries,2. To isolate single-chain antibodies to specific antigens.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA062220-11A2
Application #
6942229
Study Section
Subcommittee G - Education (NCI)
Project Start
2004-07-12
Project End
2010-03-31
Budget Start
2004-07-12
Budget End
2006-04-30
Support Year
11
Fiscal Year
2005
Total Cost
$67,742
Indirect Cost
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
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