This core will develop highly selective, high affinity antibodies to investigate coordinated cell signaling.Antibodies will be generated to distinguish active forms of target enzymes. The antibodies will be isolatedfrom an established cDNA, single-chain immune library displayed on ribosomes. This library will bescreened using modified peptides that mimic the effector regions of key signal proteins or, alternatively, fulllengthactivated proteins. This objective will be fulfilled in two specific aims:1. To maintain immune libraries,2. To isolate single-chain antibodies to specific antigens.
Showing the most recent 10 out of 253 publications