Abstract

The objective of the electron paramagnetic resonance core (EPR Core) in this research program is to provide the technical support and consultative expertise to insure success for those aspects of the research projects that require EPR spectroscopy. In addition, it is a goal of the EPR Center to develop new methods and tools that will permit the project leaders to pursue research directions that currently are not possible. The EPR Core will provide facilities and expertise to pursue experiments in the following general areas: 1. detection of free radicals generated by the systems being studied in the various projects, direct EPR detection when possible or detection using the EPR spin trapping technique; 2. the detection of nitric oxide; 3. the role of iron catalysts in free radical processes; 4. analysis of alterations in membrane biophysical properties employing EPR spin probes and fluorescent polarization techniques; and 5. development and understanding of the EPR spin trapping of lipid- derived radicals from cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA066081-03
Application #
6103028
Study Section
Project Start
1998-02-06
Project End
1999-01-31
Budget Start
Budget End
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Khoo, Nicholas K H; Hebbar, Sachin; Zhao, Weiling et al. (2013) Differential activation of catalase expression and activity by PPAR agonists: implications for astrocyte protection in anti-glioma therapy. Redox Biol 1:70-9
Carr, Wanakee J; Oberley-Deegan, Rebecca E; Zhang, Yuping et al. (2011) Antioxidant proteins and reactive oxygen species are decreased in a murine epidermal side population with stem cell-like characteristics. Histochem Cell Biol 135:293-304
Du, J; Liu, J; Smith, B J et al. (2011) Role of Rac1-dependent NADPH oxidase in the growth of pancreatic cancer. Cancer Gene Ther 18:135-43
Sun, Wenqing G; Weydert, Christine J; Zhang, Yuping et al. (2010) Superoxide Enhances the Antitumor Combination of AdMnSOD Plus BCNU in Breast Cancer. Cancers (Basel) 2:68-87
Simons, Andrean L; Mattson, David M; Dornfeld, Ken et al. (2009) Glucose deprivation-induced metabolic oxidative stress and cancer therapy. J Cancer Res Ther 5 Suppl 1:S2-6
Aykin-Burns, Nùkhet; Ahmad, Iman M; Zhu, Yueming et al. (2009) Increased levels of superoxide and H2O2 mediate the differential susceptibility of cancer cells versus normal cells to glucose deprivation. Biochem J 418:29-37
Sun, Wenqing; Kalen, Amanda L; Smith, Brian J et al. (2009) Enhancing the antitumor activity of adriamycin and ionizing radiation. Cancer Res 69:4294-300
Du, Changbin; Gao, Zhen; Venkatesha, Venkatasubbaiah A et al. (2009) Mitochondrial ROS and radiation induced transformation in mouse embryonic fibroblasts. Cancer Biol Ther 8:1962-71
Jacobs, Kristi Muldoon; Pennington, J Daniel; Bisht, Kheem S et al. (2008) SIRT3 interacts with the daf-16 homolog FOXO3a in the mitochondria, as well as increases FOXO3a dependent gene expression. Int J Biol Sci 4:291-9
Weydert, Christine J; Zhang, Yuping; Sun, Wenqing et al. (2008) Increased oxidative stress created by adenoviral MnSOD or CuZnSOD plus BCNU (1,3-bis(2-chloroethyl)-1-nitrosourea) inhibits breast cancer cell growth. Free Radic Biol Med 44:856-67

Showing the most recent 10 out of 163 publications