Abstract

The purpose of the Antioxidant Enzyme CORE (AEC) is to provide antioxidant enzyme expertise and analysis for the individual research projects. Discussion between a project investigator and the AEC Director will be held to determine which experiments and/assays to run. The AEC staff will carry out the proposed work or will advise the investigator on how to perform the experiments. Some of this work, particularly transfections in new cell lines, will be developmental in nature. The AEC will aid in enzyme assays, Western, Northern, and Southern analysis. The enzyme studies will be run on the antioxidant enzymes copper- and zinc-containing superoxide dismutase (CuZnSOD), manganese- containing superoxide dismutase (MnSOD), catalase (CAT), and glutathione peroxidase (GPX). For reagents, we have antibodies and cDNA probes to all four primary antioxidant enzymes. In addition, we will aid in transfecting the cDNAs for these enzymes into cell lines of interest to the project investigators. We have experience with several methods of transfection, as well as many different expression vectors for these enzymes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA066081-03
Application #
6103030
Study Section
Project Start
1998-02-06
Project End
1999-01-31
Budget Start
Budget End
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Khoo, Nicholas K H; Hebbar, Sachin; Zhao, Weiling et al. (2013) Differential activation of catalase expression and activity by PPAR agonists: implications for astrocyte protection in anti-glioma therapy. Redox Biol 1:70-9
Carr, Wanakee J; Oberley-Deegan, Rebecca E; Zhang, Yuping et al. (2011) Antioxidant proteins and reactive oxygen species are decreased in a murine epidermal side population with stem cell-like characteristics. Histochem Cell Biol 135:293-304
Du, J; Liu, J; Smith, B J et al. (2011) Role of Rac1-dependent NADPH oxidase in the growth of pancreatic cancer. Cancer Gene Ther 18:135-43
Sun, Wenqing G; Weydert, Christine J; Zhang, Yuping et al. (2010) Superoxide Enhances the Antitumor Combination of AdMnSOD Plus BCNU in Breast Cancer. Cancers (Basel) 2:68-87
Simons, Andrean L; Mattson, David M; Dornfeld, Ken et al. (2009) Glucose deprivation-induced metabolic oxidative stress and cancer therapy. J Cancer Res Ther 5 Suppl 1:S2-6
Aykin-Burns, Nùkhet; Ahmad, Iman M; Zhu, Yueming et al. (2009) Increased levels of superoxide and H2O2 mediate the differential susceptibility of cancer cells versus normal cells to glucose deprivation. Biochem J 418:29-37
Sun, Wenqing; Kalen, Amanda L; Smith, Brian J et al. (2009) Enhancing the antitumor activity of adriamycin and ionizing radiation. Cancer Res 69:4294-300
Du, Changbin; Gao, Zhen; Venkatesha, Venkatasubbaiah A et al. (2009) Mitochondrial ROS and radiation induced transformation in mouse embryonic fibroblasts. Cancer Biol Ther 8:1962-71
Jacobs, Kristi Muldoon; Pennington, J Daniel; Bisht, Kheem S et al. (2008) SIRT3 interacts with the daf-16 homolog FOXO3a in the mitochondria, as well as increases FOXO3a dependent gene expression. Int J Biol Sci 4:291-9
Weydert, Christine J; Zhang, Yuping; Sun, Wenqing et al. (2008) Increased oxidative stress created by adenoviral MnSOD or CuZnSOD plus BCNU (1,3-bis(2-chloroethyl)-1-nitrosourea) inhibits breast cancer cell growth. Free Radic Biol Med 44:856-67

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