The Neural Stem Cell and Neuroepithelial Tumor Isolation and Upkeep Core will coordinate maintenance of neural stem cells and neoplastic cell lines. It will also coordinate distribution of these cells to various individual projects. This core will maintain a library of frozen neurosurgical specimens for use in the various projects. Fetal, post- natal, and adult stem cells will be harvested in the core. The core will use cell culture techniques to provide these services. Because each of the projects requires the use of these cells, the core will ensure quality control of the primary research substrates of the program project. Quality control will be ensured by 1) standard cell culture techniques maintaining low passage number, 2) immunohistochemical staining, 3) RT-PCR banding patterns, and 4) timed pregnancies of the animals from which the fetal cells are harvested.
| Jang, Taichang; Savarese, Todd; Low, Hoi Pang et al. (2006) Osteopontin expression in intratumoral astrocytes marks tumor progression in gliomas induced by prenatal exposure to N-ethyl-N-nitrosourea. Am J Pathol 168:1676-85 |
| Savarese, Todd M; Jang, Taichang; Low, Hoi Pang et al. (2005) Isolation of immortalized, INK4a/ARF-deficient cells from the subventricular zone after in utero N-ethyl-N-nitrosourea exposure. J Neurosurg 102:98-108 |
| Jang, Taichang; Litofsky, N Scott; Smith, Thomas W et al. (2004) Aberrant nestin expression during ethylnitrosourea-(ENU)-induced neurocarcinogenesis. Neurobiol Dis 15:544-52 |
| Lachyankar, Mahesh B; Condon, Peter J; Daou, Marie-Claire et al. (2003) Novel functional interactions between Trk kinase and p75 neurotrophin receptor in neuroblastoma cells. J Neurosci Res 71:157-72 |
| Li, Li; Liu, Fenghua; Ross, Alonzo H (2003) PTEN regulation of neural development and CNS stem cells. J Cell Biochem 88:24-8 |
| Quesenberry, P J; Colvin, G A; Abedi, M et al. (2003) The marrow stem cell: the continuum. Bone Marrow Transplant 32 Suppl 1:S19-22 |
| Schonhoff, Christopher M; Daou, Marie-Claire; Jones, Stephen N et al. (2002) Nitric oxide-mediated inhibition of Hdm2-p53 binding. Biochemistry 41:13570-4 |
| Engstrom, Caron M; Demers, Delia; Dooner, Mark et al. (2002) A method for clonal analysis of epidermal growth factor-responsive neural progenitors. J Neurosci Methods 117:111-21 |
| Li, Li; Liu, Fenghua; Salmonsen, Rebecca A et al. (2002) PTEN in neural precursor cells: regulation of migration, apoptosis, and proliferation. Mol Cell Neurosci 20:21-9 |
| Recht, L D; Salmonsen, R; Rosetti, R et al. (2001) Antitumor effects of ajulemic acid (CT3), a synthetic non-psychoactive cannabinoid. Biochem Pharmacol 62:755-63 |
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