Abstract

The purpose of this core is to provide genetically modified animals, employing transgenic methodologies, for use in Projects 1-5. Mice will be obtained either via DNA injection of fertilized eggs or via injection of embryonic stem cells (ES cells) into blastocysts. The ES-cells carry targeted mutations obtained via homologous recombination. The core receives injection-ready DNA or ES-cells carrying targeted mutations from the different investigators and provides them with transgenic or chimeric mice for further breeding. In addition, the core will coordinate the injections and provide cell lines, guidance, advice and training to investigators to ensure efficient generation of the transgenic strains with a high success rate.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA071907-05
Application #
6318301
Study Section
Project Start
2000-06-01
Project End
2001-05-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
2000
Total Cost
$197,721
Indirect Cost

  Institution

Name
St. Jude Children's Research Hospital
Department
Type
DUNS #
067717892
City
Memphis
State
TN
Country
United States
Zip Code
38105

  Publications

Dumitrache, Lavinia C; McKinnon, Peter J (2017) Polynucleotide kinase-phosphatase (PNKP) mutations and neurologic disease. Mech Ageing Dev 161:121-129
Ribeiro, Raul C; Antillon, Federico; Pedrosa, Francisco et al. (2016) Global Pediatric Oncology: Lessons From Partnerships Between High-Income Countries and Low- to Mid-Income Countries. J Clin Oncol 34:53-61
Doherty, Joanne R; Nilsson, Lisa M; Kuliyev, Emin et al. (2014) Embryonic Expression and Function of the Xenopus Ink4d Cyclin D-Dependent Kinase Inhibitor. Cell Dev Biol 3:
Morfouace, Marie; Shelat, Anang; Jacus, Megan et al. (2014) Pemetrexed and gemcitabine as combination therapy for the treatment of Group3 medulloblastoma. Cancer Cell 25:516-29
Wang, Yuefeng; Fisher, John C; Mathew, Rose et al. (2011) Intrinsic disorder mediates the diverse regulatory functions of the Cdk inhibitor p21. Nat Chem Biol 7:214-21
Trikha, Prashant; Sharma, Nidhi; Opavsky, Rene et al. (2011) E2f1-3 are critical for myeloid development. J Biol Chem 286:4783-95
Doghman, Mabrouka; El Wakil, Abeer; Cardinaud, Bruno et al. (2010) Regulation of insulin-like growth factor-mammalian target of rapamycin signaling by microRNA in childhood adrenocortical tumors. Cancer Res 70:4666-75
Pottier, N; Paugh, S W; Ding, C et al. (2010) Promoter polymorphisms in the ?-2 adrenergic receptor are associated with drug-induced gene expression changes and response in acute lymphoblastic leukemia. Clin Pharmacol Ther 88:854-61
Huang, Danny T; Ayrault, Olivier; Hunt, Harold W et al. (2009) E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell 33:483-95
Forget, Antoine; Ayrault, Olivier; den Besten, Willem et al. (2008) Differential post-transcriptional regulation of two Ink4 proteins, p18 Ink4c and p19 Ink4d. Cell Cycle 7:3737-46

Showing the most recent 10 out of 124 publications