The Specific Aim of this Core is to provide two categories of services to the Program Project: 1) flow cytometry and cell sorting methods and technologies, and 2) assays of glutathione and the activity of redox enzymes glutathione-s-transferase, superoxide dismutase (S.O.D.) and catalase. We will also assist investigators in finding the most appropriate ways to apply these techniques to their research objectives and to interpret the experimental results. Cytometry and cell sorting will be essential parts of each of the program projects, providing variety of analytical services and techniques. Assays of DNA content, cell cycle and cell proliferation, a novel cytometric assay of oxidative DNA damage and repair, and cytometric assays related to resistance to oxidative damage (glutathione content, cell viability and apoptosis) will constitute major uses of cytometry in this Program Project proposal. In addition, this core will provide non-cytometric assays that will be widely used in the Program Project: HPLC and spectrophotometric quantitative assays of the important intracellular antioxidant glutathione; assays of glutathione-s- transferase, a principal enzyme responsible for carcinogen detoxification; assays of GSH peroxidase and GSH reductase, important enzymes in the synthesis of glutathione, and assays of the oxygen radial scavenging enzymes superoxide dismutase (S.O.D.) and catalase.

National Institute of Health (NIH)
National Cancer Institute (NCI)
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Fred Hutchinson Cancer Research Center
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Passarelli, Michael N; Newcomb, Polly A; Makar, Karen W et al. (2015) Blood lipids and colorectal polyps: testing an etiologic hypothesis using phenotypic measurements and Mendelian randomization. Cancer Causes Control 26:467-73
Adams, Scott V; Newcomb, Polly A; Burnett-Hartman, Andrea N et al. (2014) Rare circulating microRNAs as biomarkers of colorectal neoplasia. PLoS One 9:e108668
Burnett-Hartman, Andrea N; Newcomb, Polly A; Hutter, Carolyn M et al. (2014) Variation in the association between colorectal cancer susceptibility loci and colorectal polyps by polyp type. Am J Epidemiol 180:223-32
Burnett-Hartman, Andrea N; Newcomb, Polly A; Potter, John D et al. (2013) Genomic aberrations occurring in subsets of serrated colorectal lesions but not conventional adenomas. Cancer Res 73:2863-72
Burnett-Hartman, Andrea N; Passarelli, Michael N; Adams, Scott V et al. (2013) Differences in epidemiologic risk factors for colorectal adenomas and serrated polyps by lesion severity and anatomical site. Am J Epidemiol 177:625-37
Burnett-Hartman, Andrea N; Newcomb, Polly A; Phipps, Amanda I et al. (2012) Colorectal endoscopy, advanced adenomas, and sessile serrated polyps: implications for proximal colon cancer. Am J Gastroenterol 107:1213-9
Burnett-Hartman, Andrea N; Newcomb, Polly A; Mandelson, Margaret T et al. (2011) No evidence for human papillomavirus in the etiology of colorectal polyps. Cancer Epidemiol Biomarkers Prev 20:2288-97
Adams, Scott V; Newcomb, Polly A; Burnett-Hartman, Andrea N et al. (2011) Circulating 25-hydroxyvitamin-D and risk of colorectal adenomas and hyperplastic polyps. Nutr Cancer 63:319-26
Chia, Victoria M; Newcomb, Polly A; Lampe, Johanna W et al. (2007) Leptin concentrations, leptin receptor polymorphisms, and colorectal adenoma risk. Cancer Epidemiol Biomarkers Prev 16:2697-703
O'Sullivan, Jacintha; Risques, Rosa Ana; Mandelson, Margaret T et al. (2006) Telomere length in the colon declines with age: a relation to colorectal cancer? Cancer Epidemiol Biomarkers Prev 15:573-7

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