Abstract

The tissue culture Core C will supply all investigators with colorectal carcinomas cells (CRC), fibroblasts, and B-lymphocytes. The core will maintain an available bank of well-characterized cell lines of CRC and will monitor the quality of the cell lines by testing for expression of tumor-associated antigens and mycoplasma infections, and for identity by DNA fingerprinting. New permanent cell lines will be established either directly from patients' lesions or after growth of tumors in SCID mice. These immunodeficient animals will also serve to produce larger quantities of tumor material for biochemical studies. The Core C is staffed by highly experienced personnel, who are intimately familiar with all the necessary techniques and who have demonstrated expertise in the tissue culture field.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA074294-03
Application #
6300550
Study Section
Project Start
2000-01-01
Project End
2000-12-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$154,053
Indirect Cost

  Institution

Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Berencsi, Klara; Rani, Pyapalli; Zhang, Tianqian et al. (2011) In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2. J Transl Med 9:33
Swoboda, Rolf K; Somasundaram, Rajasekharan; Caputo, Laura et al. (2010) Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient. Int J Cancer 127:1124-30
Kalabis, Jiri; Li, Gang; Fukunaga-Kalabis, Mizuho et al. (2008) Endothelin-3 stimulates survival of goblet cells in organotypic cultures of fetal human colonic epithelium. Am J Physiol Gastrointest Liver Physiol 295:G1182-9
Berencsi, Klara; Meropol, Neal J; Hoffman, John P et al. (2007) Colon carcinoma cells induce CXCL11-dependent migration of CXCR3-expressing cytotoxic T lymphocytes in organotypic culture. Cancer Immunol Immunother 56:359-70
Furth, Emma E; Li, Jian; Purev, Enkhtsetseg et al. (2006) Serum antibodies to EpCAM in healthy donors but not ulcerative colitis patients. Cancer Immunol Immunother 55:528-37
Gutzmer, Ralf; Li, Wei; Sutterwala, Shaheen et al. (2004) A tumor-associated glycoprotein that blocks MHC class II-dependent antigen presentation by dendritic cells. J Immunol 173:1023-32
Birebent, Brigitte; Mitchell, Edith; Akis, Nese et al. (2003) Monoclonal anti-idiotypic antibody mimicking the gastrointestinal carcinoma-associated epitope CO17-1A elicits antigen-specific humoral and cellular immune responses in colorectal cancer patients. Vaccine 21:1601-12
Schaider, Helmut; Oka, Masahiro; Bogenrieder, Thomas et al. (2003) Differential response of primary and metastatic melanomas to neutrophils attracted by IL-8. Int J Cancer 103:335-43
Armstrong, Andrew; Eck, Stephen L (2003) EpCAM: A new therapeutic target for an old cancer antigen. Cancer Biol Ther 2:320-6
Zaloudik, Jan; Li, Weiping; Jacob, Lutz et al. (2002) Inhibition of tumor growth by recombinant vaccinia virus expressing GA733/CO17-1A/EpCAM/KSA/KS1-4 antigen in mice. Cancer Gene Ther 9:382-9

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