Abstract

The immunology Core is Designed to provide immunologic reagents needed in Project 1 through 4. These reagents are established: monoclonal antibodies (Mabs) to the human colorectal carcinoma (CRC) antigen (Ag) GA733 and the murine homolog mEGP. Polyclonal antibodies to these Ags will be purified on Ag immunoaffinity columns. New Mab to fragments of the GA733 Ag (derived from Project 1) will be generated. Antibodies from vaccinating (under Project 3) patients, healthy individuals and patients with ulcerative colitis, will be affinity-purified for detailed tissue binding specificity analysis. In addition, Core B will produce purified baculovirus-derived GA733 Ag and mEGP. A clinical grade batch of baculovirus-derived GA733 Ag will be produced for skin testing of patients in Project 3. All reagants will be tested for purity, identity, and immunoreactivity. The centralized services provided by experienced investigators will improve quality of the Ag and antibody preparations and save overall costs as compared to their inclusion as part of each project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA074294-05
Application #
6563878
Study Section
Project Start
2002-01-17
Project End
2002-12-31
Budget Start
Budget End
Support Year
5
Fiscal Year
2002
Total Cost
$228,401
Indirect Cost

  Institution

Name
Wistar Institute
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Berencsi, Klara; Rani, Pyapalli; Zhang, Tianqian et al. (2011) In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2. J Transl Med 9:33
Swoboda, Rolf K; Somasundaram, Rajasekharan; Caputo, Laura et al. (2010) Nucleophosmin is recognized by a cytotoxic T cell line derived from a rectal carcinoma patient. Int J Cancer 127:1124-30
Kalabis, Jiri; Li, Gang; Fukunaga-Kalabis, Mizuho et al. (2008) Endothelin-3 stimulates survival of goblet cells in organotypic cultures of fetal human colonic epithelium. Am J Physiol Gastrointest Liver Physiol 295:G1182-9
Berencsi, Klara; Meropol, Neal J; Hoffman, John P et al. (2007) Colon carcinoma cells induce CXCL11-dependent migration of CXCR3-expressing cytotoxic T lymphocytes in organotypic culture. Cancer Immunol Immunother 56:359-70
Furth, Emma E; Li, Jian; Purev, Enkhtsetseg et al. (2006) Serum antibodies to EpCAM in healthy donors but not ulcerative colitis patients. Cancer Immunol Immunother 55:528-37
Gutzmer, Ralf; Li, Wei; Sutterwala, Shaheen et al. (2004) A tumor-associated glycoprotein that blocks MHC class II-dependent antigen presentation by dendritic cells. J Immunol 173:1023-32
Birebent, Brigitte; Mitchell, Edith; Akis, Nese et al. (2003) Monoclonal anti-idiotypic antibody mimicking the gastrointestinal carcinoma-associated epitope CO17-1A elicits antigen-specific humoral and cellular immune responses in colorectal cancer patients. Vaccine 21:1601-12
Schaider, Helmut; Oka, Masahiro; Bogenrieder, Thomas et al. (2003) Differential response of primary and metastatic melanomas to neutrophils attracted by IL-8. Int J Cancer 103:335-43
Armstrong, Andrew; Eck, Stephen L (2003) EpCAM: A new therapeutic target for an old cancer antigen. Cancer Biol Ther 2:320-6
Zaloudik, Jan; Li, Weiping; Jacob, Lutz et al. (2002) Inhibition of tumor growth by recombinant vaccinia virus expressing GA733/CO17-1A/EpCAM/KSA/KS1-4 antigen in mice. Cancer Gene Ther 9:382-9

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