Abstract

The purpose of Core B, the In Vivo Experimentation Core (IVEC) is to provide logistical support, expertise and guidance for examining Ets gene function in vivo by using the mouse as model organism. This core consists of five components, including, (i) maintenance and husbandry of transgenic mouse strains, (ii) generation of new transgenic models, (iii) morphometrical and histological analysis of mammary gland (iv) cryopreservation, and (v) reporter gene analysis. This core structure ensures access to state-of-the-art technology to for studying mammary gland tumor development and metastasis in existing and new transgenic mouse models. Centralizing in vivo experimentation has several distinct advantages over individualized experimentation, including greater efficiency, uniformity of experimental conditions (which facilitates direct data comparison), providing a source of inspiration for developing plans or hypotheses for cross-project experimentation (e.g. crossing of mutant strains), and rapid adaptation to emerging new methodologies. Consequently, this core will be a dynamic unit with maximal responsiveness to the needs of the individual projects both at present, and as they arise during their progress.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA078582-10
Application #
7803626
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2009-05-01
Budget End
2010-04-30
Support Year
10
Fiscal Year
2009
Total Cost
$255,633
Indirect Cost

  Institution

Name
Medical University of South Carolina
Department
Type
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425

  Publications

Scheiber, Melissa N; Watson, Patricia M; Rumboldt, Tihana et al. (2014) FLI1 expression is correlated with breast cancer cellular growth, migration, and invasion and altered gene expression. Neoplasia 16:801-13
Kornblau, Steven M; Qiu, Yi Hua; Zhang, Nianxiang et al. (2011) Abnormal expression of FLI1 protein is an adverse prognostic factor in acute myeloid leukemia. Blood 118:5604-12
Turner, David P; Findlay, Victoria J; Moussa, Omar et al. (2011) Mechanisms and functional consequences of PDEF protein expression loss during prostate cancer progression. Prostate 71:1723-35
Markiewicz, Margaret; Nakerakanti, Sashidhar S; Kapanadze, Bagrat et al. (2011) Connective tissue growth factor (CTGF/CCN2) mediates angiogenic effect of S1P in human dermal microvascular endothelial cells. Microcirculation 18:1-11
Findlay, Victoria J; Turner, David P; Yordy, John S et al. (2011) Prostate-Derived ETS Factor Regulates Epithelial-to-Mesenchymal Transition through Both SLUG-Dependent and Independent Mechanisms. Genes Cancer 2:120-9
Duchi, Serena; Fagnocchi, Luca; Cavaliere, Valeria et al. (2010) Drosophila VHL tumor-suppressor gene regulates epithelial morphogenesis by promoting microtubule and aPKC stability. Development 137:1493-503
Asano, Yoshihide; Stawski, Lukasz; Hant, Faye et al. (2010) Endothelial Fli1 deficiency impairs vascular homeostasis: a role in scleroderma vasculopathy. Am J Pathol 176:1983-98
Moussa, Omar; LaRue, Amanda C; Abangan Jr, Romeo S et al. (2010) Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1. Mol Cell Biol 30:5194-206
Elkareh, Jihad; Periyasamy, Sankaridrug M; Shidyak, Amjad et al. (2009) Marinobufagenin induces increases in procollagen expression in a process involving protein kinase C and Fli-1: implications for uremic cardiomyopathy. Am J Physiol Renal Physiol 296:F1219-26
Shirai, Keisuke; Sera, Yasuhiko; Bulkeley, William et al. (2009) Hematopoietic stem cell origin of human fibroblasts: cell culture studies of female recipients of gender-mismatched stem cell transplantation and patients with chronic myelogenous leukemia. Exp Hematol 37:1464-71

Showing the most recent 10 out of 84 publications