Abstract

The Laboratory Services Core will support projects by providing services in three areas. These areas are: production and characterization of purified Vectors; production of characterized cells; and pathology studies. Vector production and characterization will be consolidated in the core to avoid duplicative efforts, provide uniform lots and apply standard protocols based on good laboratory practices. Production of vectors and cells in the core will be supervised by personnel with extensive experience in virus production and quality control testing of biological products. The core will maintain characterized cell banks for vector production and functional assays. Cells produced by the core will be used to analyze the tumorigenicity and sensitivity to adenovirus infectivity in vitro and in vivo. The core will be responsible for testing viral based vector lots for replication compentent adenoviruses (RCA). Vectors will also be tested for endotoxin. Both vector lots and cell lines supplied to core users will be tested for adventitious microbial agents, including mycoplasma. Pathology Studies. The core will support projects by providing pathology services, including assays focused on the analysis of apoptosis such as the TUNEL assay, morphological analysis and standard pathology for tissue analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
1P01CA078778-01A1
Application #
6232840
Study Section
Subcommittee G - Education (NCI)
Project Start
1999-07-23
Project End
2004-04-30
Budget Start
Budget End
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Texas MD Anderson Cancer Center
Department
Type
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Pataer, Apar; Kalhor, Neda; Correa, Arlene M et al. (2012) Histopathologic response criteria predict survival of patients with resected lung cancer after neoadjuvant chemotherapy. J Thorac Oncol 7:825-32
Kadowaki, Yoshihiko; Chari, Nikhil S; Teo, Albert E K et al. (2011) PI3 Kinase inhibition on TRAIL-induced apoptosis correlates with androgen-sensitivity and p21 expression in prostate cancer cells. Apoptosis 16:627-35
Pataer, Abujiang; Swisher, Stephen G; Roth, Jack A et al. (2009) Inhibition of RNA-dependent protein kinase (PKR) leads to cancer cell death and increases chemosensitivity. Cancer Biol Ther 8:245-52
Pataer, Abujiang; Chada, Sunil; Roth, Jack A et al. (2008) Development of Ad-mda7/IL-24-resistant lung cancer cell lines. Cancer Biol Ther 7:103-8
Pataer, Abujiang; Hu, Wenxian; Xiaolin, Lu et al. (2008) Adenoviral endoplasmic reticulum-targeted mda-7/interleukin-24 vector enhances human cancer cell killing. Mol Cancer Ther 7:2528-35
Pataer, A; Bocangel, D; Chada, S et al. (2007) Enhancement of adenoviral MDA-7-mediated cell killing in human lung cancer cells by geldanamycin and its 17-allyl- amino-17-demethoxy analogue. Cancer Gene Ther 14:12-8
Peng, Henry H; Wu, Shuhong; Davis, John J et al. (2006) A rapid and efficient method for purification of recombinant adenovirus with arginine-glycine-aspartic acid-modified fibers. Anal Biochem 354:140-7
Pataer, A; Fanale, M A; Roth, J A et al. (2006) Induction of apoptosis in human lung cancer cells following treatment with amifostine and an adenoviral vector containing wild-type p53. Cancer Gene Ther 13:806-14
Li, Zhongkui; Niu, Jiangong; Uwagawa, Tadashi et al. (2005) Function of polo-like kinase 3 in NF-kappaB-mediated proapoptotic response. J Biol Chem 280:16843-50
Sclabas, Guido M; Fujioka, Shuichi; Schmidt, Christian et al. (2005) Overexpression of tropomysin-related kinase B in metastatic human pancreatic cancer cells. Clin Cancer Res 11:440-9

Showing the most recent 10 out of 88 publications