A hallmark of our laboratory has been the development of novel and innovative in vivo tumor models. These models have allowed is to study barriers to the delivery of therapeutic agents. This has been possible due to the outstanding surgical expertise and unique facility available in our lab. The proposed core builds on this foundation. This core will serve two functions: (i) surgical support, and (ii) breeding and maintenance of animals. Both are vital for successful completion of all four projects. Thus, this core forms the cornerstone of the proposed PPG. The following animal models have been established in our laboratory and will provided by this core. Transparent window models (all four projects) will enable us to monitor physiological parameters and gene regulation in vivo. The liver tumor (Project 1 and 3) and mammary fat pad tumor (Project 4) models will allow us to study the role of host microenvironment. The micropore chamber model (Project 2) will allow us to collect interstitial fluid. Tissue sample collection, control release pump implantation, vascular line placement, and post-surgical care will also be available through this core. The gnotobiotic animal colony in this core will allow us to carry out longitudinal physiological studies in immunodeficient mice. These studies are extremely difficult and more costly and more costly elsewhere. This facility will continue to provide us uniform quality experimental animals free of murine viruses, pathogenic bacteria, and parasites. This will enable us to carry out surgical procedures without antibiotics Dined flora C3H (Project 4), SCID (Project 2, 3, 4) mice and RAG-2 knock out (KO) (Project 1, 3, 4) mice are currently available. In addition, GFP transgenic mice developed in Project 1, VEGF conditional KO and bFGF KO mice (Project 1), decorin KO mice (Project 3) and scavenger receptor KO mice (Project 4) will be back crossed with Rag-2 KO mice to obtain immunodeficient background and will be bred and maintained by this Core. Tumors screened and free of mouse pathogens will be serially passaged in vivo and implanted into experimental animals. In vivo tumors are not passaged beyond the fifth generation to avoid changes in tumor characteristics. This assures tight quality control of animals and tumors.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
1P01CA080124-01A1
Application #
6402413
Study Section
Subcommittee E - Prevention &Control (NCI)
Project Start
2000-08-11
Project End
2005-07-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2000
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
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