Services provided by the Tissue Analysis Core Laboratory are technical and professional.The Core is vital to theProgram Project as all of the individual Projects will utilize the services and resources of the Core. The servicesinclude:
Aim 1. Collection and analysis of Prostate Tumor tissue and cellular samples.
Aim 1. 1) the continued collection of frozen normal and neoplastic prostate tissue specimens from radicalprostatectomies in an appropriate manner to ensure optimal nucleic acid and protein preservation. This isperformed in a manner such that patient care is not compromised.
Aim 1. 2) the performance of routine histology on cell culture, xenograft, mouse models, and clinical humanprostate tissue specimens. In addition, Dr. Frierson will use his expertise as a pathologist to examine theprostate glands of transgenic mice to determine histopathologic changes induced by the genetic modifications.
Aim 1. 3) the performance of immunohistochemistry and the characterization of antibodies, to determine theirutility in frozen tissue sections and in formalin-fixed, paraffin-embedded material with and without usingmethods of epitope retrieval. Dr. Frierson will use his immunohistochemical expertise to determine optimalreagent conditions and antibody staining in cell culture, xenograft, mouse models, and clinical human prostatecancer specimensAim 1.4) selection, preparation, and procurement of cells for laser microdissection. In prostates of transgenicmice, Dr. Frierson will perform laser microdissection of selected cells for molecular analysis.
Aim 2. Bone histology and histomorphometry will be performed on xenograft mouse models of prostatecancer. Bone samples will be processed and analyzed by Dr. Guise to assess the bone metastases phenotypeand to quantitate tumor burden, and osteoclast and osteoblast activity.All of the data generated by CORE C will be submitted to the PROSTA interactive searchable database that willbe designed by CORE A. Submission of data from CORE C to this common data repository and retrieval systemwill better integrate the programmatic interactions between CORE C and all Projects and Cores.
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