Abstract

The Fusion Proteins and Monoclonal Antibodies Core will design, generate and purify fusion proteins andmonoclonal antibodies for the Regulation of Tumor Immunity Program. The core in a cost-effective way willprovide individual projects with large amounts of monoclonal antibodies and fusion proteins needed for platecoating, large scale cell separation in vitro, and cell blocking and depletion experiments in vivo. The core willalso purify required amounts of monoclonal antibodies and fusion proteins unique for the proposed research,and generate and characterize new fusion proteins and hybridomas.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
1P01CA109094-01A2
Application #
7226415
Study Section
Special Emphasis Panel (ZCA1-RPRB-7 (O5))
Project Start
2006-12-01
Project End
2012-03-31
Budget Start
2007-04-20
Budget End
2008-03-31
Support Year
1
Fiscal Year
2007
Total Cost
$80,929
Indirect Cost

  Institution

Name
University of Miami School of Medicine
Department
Type
DUNS #
052780918
City
Coral Gables
State
FL
Country
United States
Zip Code
33146

  Publications

Wolf, Dietlinde; Barreras, Henry; Bader, Cameron S et al. (2017) Marked in Vivo Donor Regulatory T Cell Expansion via Interleukin-2 and TL1A-Ig Stimulation Ameliorates Graft-versus-Host Disease but Preserves Graft-versus-Leukemia in Recipients after Hematopoietic Stem Cell Transplantation. Biol Blood Marrow Transplant 23:757-766
Schwartz, Marc; Zhang, Yu; Rosenblatt, Joseph D (2016) B cell regulation of the anti-tumor response and role in carcinogenesis. J Immunother Cancer 4:40
McCormack, Ryan M; Lyapichev, Kirill; Olsson, Melissa L et al. (2015) Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2. Elife 4:
McCormack, Ryan M; de Armas, Lesley R; Shiratsuchi, Motoaki et al. (2015) Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. Elife 4:
Hatfield, Stephen M; Kjaergaard, Jorgen; Lukashev, Dmitriy et al. (2015) Immunological mechanisms of the antitumor effects of supplemental oxygenation. Sci Transl Med 7:277ra30
Newman, Robert G; Dee, Michael J; Malek, Thomas R et al. (2014) Heat shock protein vaccination and directed IL-2 therapy amplify tumor immunity rapidly following bone marrow transplantation in mice. Blood 123:3045-55
Gonzalez, Louis; Strbo, Natasa; Podack, Eckhard R (2013) Humanized mice: novel model for studying mechanisms of human immune-based therapies. Immunol Res 57:326-34
McCormack, Ryan; de Armas, Lesley R; Shiratsuchi, Motoaki et al. (2013) Inhibition of intracellular bacterial replication in fibroblasts is dependent on the perforin-like protein (perforin-2) encoded by macrophage-expressed gene 1. J Innate Immun 5:185-94
Fields, K A; McCormack, R; de Armas, L R et al. (2013) Perforin-2 restricts growth of Chlamydia trachomatis in macrophages. Infect Immun 81:3045-54
McCormack, Ryan; de Armas, Lesley; Shiratsuchi, Motoaki et al. (2013) Killing machines: three pore-forming proteins of the immune system. Immunol Res 57:268-78

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