The primary mission of the Gastric Histopathology Core is to provide high quality, rapid-turnaround specialized research histology services, including immunohistochemistry, to investigators in this PPG, and to provide expert histopathologic interpretation, through collaboration with Dr. M. Blanca Piazuelo and Dr. Kay Washington. The following services are proposed: 1. To provide expert evaluation of histopathology of rodent models of H. pylori-induced gastric neoplasia and correlation with human disease 2. To provide expertise in developing, performing and evaluating immunohistochemical studies and other special staining techniques on animal and human tissues, with robust quality control measures, and with extension to gastroids, as needed 3. To provide tissue microarray services and access to human gastric tissue samples with pathological annotation and clinical outcome. The three component projects and Core B (Proteomics and Metabolomics Core) of this proposed Program Project all rely heavily upon morphologic analysis of gastric tissues isolated from rodent models of Helicobacter pylori-induced gastritis and gastric neoplasia Core A will coordinate tissue annotation for imaging mass spectrometry and will use IHC to localize protein targets identified by Core B. Dr. Piazuelo's and Dr. Washington's expertise in interpretation of histopathologic changes in rodent models and in interpretation of immunohistochemical studies will be critical to this work, and these collaborative activities are currently not funded through existing resources. By utilizing the resources of a GI research-related immunohistochemistry facility, it will be possible to achieve high standards for all histologic studies proposed in this grant. This centralization of histologic services under the supervision of experienced pathologists with a dedicated interest in gastrointestinal pathology will allow careful attention to quality assurance. !
Core A Narrative The work in this Core will help identify factors important in gastric carcinogenesis through the use of specialized histology services. This may help identify persons at high risk for gastric cancer who can then be treated for H. pylori. !
|Singh, Kshipra; Coburn, Lori A; Asim, Mohammad et al. (2018) Ornithine Decarboxylase in Macrophages Exacerbates Colitis and Promotes Colitis-Associated Colon Carcinogenesis by Impairing M1 Immune Responses. Cancer Res 78:4303-4315|
|Corley, Douglas A; Peek Jr, Richard M (2018) When Should Guidelines Change? A Clarion Call for Evidence Regarding the Benefits and Risks of Screening for Colorectal Cancer at Earlier Ages. Gastroenterology 155:947-949|
|Gobert, Alain P; Al-Greene, Nicole T; Singh, Kshipra et al. (2018) Distinct Immunomodulatory Effects of Spermine Oxidase in Colitis Induced by Epithelial Injury or Infection. Front Immunol 9:1242|
|Raghunathan, Krishnan; Foegeding, Nora J; Campbell, Anne M et al. (2018) Determinants of Raft Partitioning of the Helicobacter pylori Pore-Forming Toxin VacA. Infect Immun 86:|
|Scoville, Elizabeth A; Allaman, Margaret M; Brown, Caroline T et al. (2018) Alterations in Lipid, Amino Acid, and Energy Metabolism Distinguish Crohn's Disease from Ulcerative Colitis and Control Subjects by Serum Metabolomic Profiling. Metabolomics 14:|
|Sierra, Johanna C; Asim, Mohammad; Verriere, Thomas G et al. (2018) Epidermal growth factor receptor inhibition downregulates Helicobacter pylori-induced epithelial inflammatory responses, DNA damage and gastric carcinogenesis. Gut 67:1247-1260|
|Blosse, Alice; Lehours, Philippe; Wilson, Keith T et al. (2018) Helicobacter: Inflammation, immunology, and vaccines. Helicobacter 23 Suppl 1:e12517|
|Coburn, Lori A; Singh, Kshipra; Asim, Mohammad et al. (2018) Loss of solute carrier family 7 member 2 exacerbates inflammation-associated colon tumorigenesis. Oncogene :|
|Loh, John T; Beckett, Amber C; Scholz, Matthew B et al. (2018) High-Salt Conditions Alter Transcription of Helicobacter pylori Genes Encoding Outer Membrane Proteins. Infect Immun 86:|
|Noto, Jennifer M; Chopra, Abha; Loh, John T et al. (2018) Pan-genomic analyses identify key Helicobacter pylori pathogenic loci modified by carcinogenic host microenvironments. Gut 67:1793-1804|
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