The Proteomics and Metabolomics Core (Core B) provides state-of-the-art instrumentation, methodology, and expertise in analytical proteomics and metabolomics to investigators in this PPG. A unique feature of the Proteomics and Metabolomics Core is the availability of cutting-edge quantitative proteomics analysis technologies that allows high sensitivity measurements of proteome changes in models of gastric cancer. New services in this renewal application include discovery and targeted metabolomics as well as tissue imaging mass spectrometry (IMS). The Core provides 1) identification and quantitation of proteins in complex samples, 2) analyses of protein modifications, including phosphorylation, 3) quantitative analyses of metabolite abundances, and 4) molecular imaging of gastric tissue using IMS. Core staff provide consultation on experimental design as well as hands on sample preparation, mass spectrometry analysis and primary data analysis. Multiple high performance mass spectrometers for LC- MS/MS experiments are available for proposed analyses. In addition, advanced methods are available including: multidimensional LC-MS/MS for protein identification from complex samples, TagRecon database searching analysis for identification of unanticipated modifications or sequence variants from MS/MS data, and high spatial and mass resolution imaging methods for tissue analysis. The Core also provides relative quantitation using stable isotope differential labeling strategies, iTRAQ and SILAC, for samples derived from animal models or from cell culture, respectively. Validation of protein expression or metabolite abundance changes is accomplished using targeted multiple reaction monitoring LC-MS/MS methods. Project 1 (Peek) will utilize metabolomics methods to measure metabolite changes in H. pylori strains isolated from infected iron deplete or replete gerbils as a function of time. Project 2 (Wilson) will utilize phospho-SILAC to measure changes in the phosphoproteome and metabolomics to measure metabolite changes in macrophages upon H. pylori infection and with EGFR or DHPS inhibition. Project 3 (Cover) will employ metabolomics methods to examine metabolite changes in plasma and gastric tissue. In addition, this project will utilize IMS methods to measure changes in metabolites, proteins and lipids in gastric tissue in a spatially- resolved manner as a function of dietary salt, infection and inflammation. !

Public Health Relevance

Core B Narrative The use of state-of-the-art proteomics, metabolomics and imaging mass spectrometry technologies in the three Projects will identify protein, lipid, and metabolite changes in gastric cells, macrophages and bacterial cells that play important roles in gastric cancer. !

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
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Special Emphasis Panel (ZCA1)
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Vanderbilt University Medical Center
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Corley, Douglas A; Peek Jr, Richard M (2018) When Should Guidelines Change? A Clarion Call for Evidence Regarding the Benefits and Risks of Screening for Colorectal Cancer at Earlier Ages. Gastroenterology 155:947-949
Gobert, Alain P; Al-Greene, Nicole T; Singh, Kshipra et al. (2018) Distinct Immunomodulatory Effects of Spermine Oxidase in Colitis Induced by Epithelial Injury or Infection. Front Immunol 9:1242
Raghunathan, Krishnan; Foegeding, Nora J; Campbell, Anne M et al. (2018) Determinants of Raft Partitioning of the Helicobacter pylori Pore-Forming Toxin VacA. Infect Immun 86:
Scoville, Elizabeth A; Allaman, Margaret M; Brown, Caroline T et al. (2018) Alterations in Lipid, Amino Acid, and Energy Metabolism Distinguish Crohn's Disease from Ulcerative Colitis and Control Subjects by Serum Metabolomic Profiling. Metabolomics 14:
Sierra, Johanna C; Asim, Mohammad; Verriere, Thomas G et al. (2018) Epidermal growth factor receptor inhibition downregulates Helicobacter pylori-induced epithelial inflammatory responses, DNA damage and gastric carcinogenesis. Gut 67:1247-1260
Blosse, Alice; Lehours, Philippe; Wilson, Keith T et al. (2018) Helicobacter: Inflammation, immunology, and vaccines. Helicobacter 23 Suppl 1:e12517
Coburn, Lori A; Singh, Kshipra; Asim, Mohammad et al. (2018) Loss of solute carrier family 7 member 2 exacerbates inflammation-associated colon tumorigenesis. Oncogene :
Loh, John T; Beckett, Amber C; Scholz, Matthew B et al. (2018) High-Salt Conditions Alter Transcription of Helicobacter pylori Genes Encoding Outer Membrane Proteins. Infect Immun 86:
Noto, Jennifer M; Chopra, Abha; Loh, John T et al. (2018) Pan-genomic analyses identify key Helicobacter pylori pathogenic loci modified by carcinogenic host microenvironments. Gut 67:1793-1804
Butt, Julia; Blot, William J; Teras, Lauren R et al. (2018) Antibody Responses to Streptococcus Gallolyticus Subspecies Gallolyticus Proteins in a Large Prospective Colorectal Cancer Cohort Consortium. Cancer Epidemiol Biomarkers Prev 27:1186-1194

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