The Lung Pathology and Molecular Analysis Core (Core B) provides a powerful combination of experiencedand capable personnel whose primary role is to provide state of the art service and support to the researchinvestigators. To do this, the Core provides the technology and technical expertise to effectively supplementthe studies in lung cancer pathology, protein analysis, and microscopy described in this proposal. The majorobjectives of this core are to (1) facilitate the acquisition, preservation, analysis, and dispersal of clinicalsamples, (2) provide accurate histopathologic characterization of tumor tissues for all project investigators,(3) allow access to state-of-the-art analytic protein chemistry and proteomics resources for use in theidentification, quantitation, and detailed structural characterization of proteins and protein complexes, and (4)provide cutting-edge microscopy and imaging tools to ensure optimal visualization and analysis of cellularprocesses. Specifically, Lung Pathology will develop and maintain a large repository of well-preserved, well-characterizedtissue specimens obtained from lung cancer patients that are receiving care or evaluation atEmory University, or are participating in the Neoadjuvant Trial of Chemotherapy Hope (NATCH), to developunbiased comprehensive histologic characterization of the tissue samples used in each of the P-01 projects.This Core also offers reliable centralized laboratory services to the investigators with respect to tissuesamples including state-of-the-art examination of immunohistochemical stains, in-situ hybridization microdissection,and other techniques. It also serves as an important central database management resource tomaintain a comprehensive prospective, interactive database to provide access to patient demographic dataas well as detailed clinical pathologic data from patients with lung cancer. The protein analysis branch willprovide critical access to state-of-the-art analytical protein chemistry and proteomics resources for use in theidentification, quantitation, and detailed structural characterization of proteins and protein complexes asneeded. Lastly, the imaging facility will provide the necessary microscopy tools and technical expertise tovisualize the biological processes described in this application. Moreover, it will employ sophisticated imageanalysis tools for image processing and accurate quantitative analyses. Taken together, the Lung Pathologyand Molecular Analysis Core serves as a critical resource that will facilitate scientific advancement throughlung pathology, protein-based initiatives, and microscopic analysis of cellular signaling pathways.
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