The In Vivo Study Support Core will develop and test the methods (imaging and histopathology) that will be used in all 5 projects to evaluate the in vivo functionality and accuracy of the minimally invasive cancer treatments developed in this PO1. Analysis of the macroscopic and microscopic pathology in post-treatment target tissues will be performed by this core in order to provide the critical feedback - paramount to the success of these minimally invasive clinical applications. The core will optimize MRI post - treatment imaging, and develop methods for registration between MR and histopathology images. For quality correlation of gross as well as macro, microscopic, and MR Images, this core will customize target-tissue specific lesion sampling, fixation, processing, embedding, slide preparation, and interpretation of the histopathology for all animal models used in this PO1. Furthermore, accuracy of correlation between MRI and histopathology will be accomplished by placement of fiducial markers in target tissues, digitization of histological slides, and registration of the MR images with digital gross and microscopic pathology images. These techniques will enable correction of lesion measurements due to tissue fixation shrinkage, and distortion of tissues due to manipulation and processing. In addition, we will incorporate special chemical and immunohistochemical stains, as needed, to detect markers of acute cell death and, degrees of tissue damage, in order to improve interpretation of thermal lesion appearance via the various acute post-treatment MR imaging modalities. Statistical support will also be provided by this core. The four Specific Aims of Core A are to:
Aim 1 : Provide Imaging for Immediate Post-Ablation Assessment of Thermal and Cryoablation Lesions.
Aim 2 : Provide Methods for Correlating MR Imaging and Pathology Slides.
Aim 3 : Provide Methods for Enhanced Pathologic Analysis of Thermal and Cryoablation Lesions.
Aim 4 : Provide Statistical Support for Data Generated in Projects 1-5 and Cores A and B.
Real time MRI post-treatment monitoring is an essential tool to accurately visualize and document the exact location of thermal and cryoablation lesions, as well as the extent of tissue damage created by these treatments. The proposed aims of Core A will enable clinicians to adjust and re-direct treatment, interpret regional target tissue viability using the real-time MR imaging modalities, and better understand the appearance of acutely dead tissues (effectively treated) vs sub-lethally damaged (ineffectively treated) tissue.
|Weber, Hans; Hargreaves, Brian A; Daniel, Bruce L (2018) Artifact-reduced imaging of biopsy needles with 2D multispectral imaging. Magn Reson Med 80:655-661|
|Webb, Taylor D; Leung, Steven A; Rosenberg, Jarrett et al. (2018) Measurements of the Relationship Between CT Hounsfield Units and Acoustic Velocity and How It Changes With Photon Energy and Reconstruction Method. IEEE Trans Ultrason Ferroelectr Freq Control 65:1111-1124|
|Weber, Hans; Ghanouni, Pejman; Pascal-Tenorio, Aurea et al. (2018) MRI monitoring of focused ultrasound sonications near metallic hardware. Magn Reson Med 80:259-271|
|Zheng, Yuan; Marx, Michael; Miller, G Wilson et al. (2018) High sensitivity MR acoustic radiation force imaging using transition band balanced steady-state free precession. Magn Reson Med 79:1532-1537|
|Han, Amy Kyungwon; Bae, Jung Hwa; Gregoriou, Katerina C et al. (2018) MR-Compatible Haptic Display of Membrane Puncture in Robot-Assisted Needle Procedures. IEEE Trans Haptics :|
|Gibbons, Eric K; Le Roux, Patrick; Pauly, John M et al. (2018) Slice profile effects on nCPMG SS-FSE. Magn Reson Med 79:430-438|
|Dixit, Neerav; Stang, Pascal P; Pauly, John M et al. (2018) Thermo-Acoustic Ultrasound for Detection of RF-Induced Device Lead Heating in MRI. IEEE Trans Med Imaging 37:536-546|
|Gibbons, Eric K; Le Roux, Patrick; Vasanawala, Shreyas S et al. (2018) Robust Self-Calibrating nCPMG Acquisition: Application to Body Diffusion-Weighted Imaging. IEEE Trans Med Imaging 37:200-209|
|Aggarwal, Kamal; Joshi, Kiran R; Rajavi, Yashar et al. (2017) A Millimeter-Wave Digital Link for Wireless MRI. IEEE Trans Med Imaging 36:574-583|
|Ghanouni, Pejman; Kishore, Sirish; Lungren, Matthew P et al. (2017) Treatment of Low-Flow Vascular Malformations of the Extremities Using MR-Guided High Intensity Focused Ultrasound: Preliminary Experience. J Vasc Interv Radiol 28:1739-1744|
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