The Recombineering and Virus Production core will be responsible for 1) recombinant virus design consultation, 2) vector and viral reagent repository;3) generation of site-directed mutations in Bacmid KSHV, and 4) large scale production of recombinant and wild-type KSHV. These goals will be accomplished by a centralized laboratory, providing a set of standardized services, including state ofthe art methods in herpes virus, molecular engineering and recombination-based mutagenesis (i.e. Kan-SacB, Kan-rpsL, Gal-K, and LoxP-kan for conditional knock-outs), production of recombinant Lenti-virus expression vectors for cDNA and shRNA, and production of KSHV from transfected 293 cells (recombinant viruses), GFP tagged viruses with specific, mutations, or BCBL1 cells (wild-type virus). Since it is expected that this program project will generate unique resources for research, the core will be responsible for storage of recombinant viruses, bacmids, reagents for recombineering and Lenti-virus production, as well as management of adequate databases that users can access and search via a web-based browser. Expertise in all aspects of protein and virus production will be provided to the projects for the lifetime of grant support.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA174439-02
Application #
8754028
Study Section
Special Emphasis Panel (ZCA1-RPRB-2)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
2
Fiscal Year
2014
Total Cost
$233,353
Indirect Cost
$49,176
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Pei, Yonggang; Singh, Rajnish Kumar; Shukla, Sanket Kumar et al. (2018) Epstein-Barr Virus Nuclear Antigen 3C Facilitates Cell Proliferation by Regulating Cyclin D2. J Virol 92:
Tso, For Yue; Kossenkov, Andrew V; Lidenge, Salum J et al. (2018) RNA-Seq of Kaposi's sarcoma reveals alterations in glucose and lipid metabolism. PLoS Pathog 14:e1006844
Mawhinney, Matthew T; Liu, Runcong; Lu, Fang et al. (2018) CTCF-Induced Circular DNA Complexes Observed by Atomic Force Microscopy. J Mol Biol 430:759-776
Li, Yuqing; Zhong, Canrong; Liu, Dawei et al. (2018) Evidence for Kaposi Sarcoma Originating from Mesenchymal Stem Cell through KSHV-induced Mesenchymal-to-Endothelial Transition. Cancer Res 78:230-245
Pandey, Saurabh; Robertson, Erle S (2018) Oncogenic Epstein-Barr virus recruits Nm23-H1 to regulate chromatin modifiers. Lab Invest 98:258-268
Liu, Dongcheng; Wang, Yan; Yuan, Yan (2018) Kaposi's Sarcoma-Associated Herpesvirus K8 Is an RNA Binding Protein That Regulates Viral DNA Replication in Coordination with a Noncoding RNA. J Virol :
Pandey, Saurabh; Jha, Hem Chandra; Shukla, Sanket Kumar et al. (2018) Epigenetic Regulation of Tumor Suppressors by Helicobacter pylori Enhances EBV-Induced Proliferation of Gastric Epithelial Cells. MBio 9:
Lang, Fengchao; Sun, Zhiguo; Pei, Yonggang et al. (2018) Shugoshin 1 is dislocated by KSHV-encoded LANA inducing aneuploidy. PLoS Pathog 14:e1007253
Aneja, Kawalpreet K; Yuan, Yan (2017) Reactivation and Lytic Replication of Kaposi's Sarcoma-Associated Herpesvirus: An Update. Front Microbiol 8:613
Chen, Horng-Shen; De Leo, Alessandra; Wang, Zhuo et al. (2017) BET-Inhibitors Disrupt Rad21-Dependent Conformational Control of KSHV Latency. PLoS Pathog 13:e1006100

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