The Molecular Analysis of Genome Instability Core will perform cytological assays to detect chromosome rearrangements, and assays to detect resection of double-strand breaks (DSBs) for program project participants. The Core Directors will consult with the program project investigators in the development of new procedures within our areas of expertise. By centralizing services under the leadership of two experts in chromosome cytology and DNA end resection, the Core will enhance the productivity of the program project members and promote synergy among the projects by providing access to critical methodologies and reagents. To accomplish these goals. Core B will pursue the following specofoc aims:
Specific Aim 1 : To provide services to analyze DNA end resection by direct physical methods.
Specific Aim 2 : To perform molecular cytogenetic assays to characterize chromosomal changes in cells derived from in vitro and in vivo experimental models.
The Molecular Analysis of Genomic Instability Core will carry out assays to detect DNA end resection and chromosome translocations, allowing Project Leaders to use their staff and facilities more efficiently and advance the overall goal ofthe Program to understand how DNA end resection and translocations contribute to cancer.
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|Gnügge, Robert; Oh, Julyun; Symington, Lorraine S (2018) Processing of DNA Double-Strand Breaks in Yeast. Methods Enzymol 600:1-24|
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|Gnügge, Robert; Symington, Lorraine S (2017) Keeping it real: MRX-Sae2 clipping of natural substrates. Genes Dev 31:2311-2312|
|Liu, Xiangyu; Shao, Zhengping; Jiang, Wenxia et al. (2017) PAXX promotes KU accumulation at DNA breaks and is essential for end-joining in XLF-deficient mice. Nat Commun 8:13816|
|Kato, Niyo; Kawasoe, Yoshitaka; Williams, Hannah et al. (2017) Sensing and Processing of DNA Interstrand Crosslinks by the Mismatch Repair Pathway. Cell Rep 21:1375-1385|
|Aparicio, Tomas; Gautier, Jean (2016) BRCA1-CtIP interaction in the repair of DNA double-strand breaks. Mol Cell Oncol 3:e1169343|
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