The Molecular Analysis of Genome Instability Core will perform cytological assays to detect chromosome rearrangements, and assays to detect resection of double-strand breaks (DSBs) for program project participants. The Core Directors will consult with the program project investigators in the development of new procedures within our areas of expertise. By centralizing services under the leadership of two experts in chromosome cytology and DNA end resection, the Core will enhance the productivity of the program project members and promote synergy among the projects by providing access to critical methodologies and reagents. To accomplish these goals. Core B will pursue the following specofoc aims:
Specific Aim 1 : To provide services to analyze DNA end resection by direct physical methods.
Specific Aim 2 : To perform molecular cytogenetic assays to characterize chromosomal changes in cells derived from in vitro and in vivo experimental models.

Public Health Relevance

The Molecular Analysis of Genomic Instability Core will carry out assays to detect DNA end resection and chromosome translocations, allowing Project Leaders to use their staff and facilities more efficiently and advance the overall goal ofthe Program to understand how DNA end resection and translocations contribute to cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
1P01CA174653-01A1
Application #
8608850
Study Section
Special Emphasis Panel ()
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
1
Fiscal Year
2014
Total Cost
$123,317
Indirect Cost
$46,244
Name
Columbia University
Department
Type
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032
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Oh, Julyun; Lee, So Jung; Rothstein, Rodney et al. (2018) Xrs2 and Tel1 Independently Contribute to MR-Mediated DNA Tethering and Replisome Stability. Cell Rep 25:1681-1692.e4
Billing, David; Horiguchi, Michiko; Wu-Baer, Foon et al. (2018) The BRCT Domains of the BRCA1 and BARD1 Tumor Suppressors Differentially Regulate Homology-Directed Repair and Stalled Fork Protection. Mol Cell 72:127-139.e8
Schrank, Benjamin R; Aparicio, Tomas; Li, Yinyin et al. (2018) Nuclear ARP2/3 drives DNA break clustering for homology-directed repair. Nature 559:61-66
Gnügge, Robert; Oh, Julyun; Symington, Lorraine S (2018) Processing of DNA Double-Strand Breaks in Yeast. Methods Enzymol 600:1-24
Crowe, Jennifer L; Shao, Zhengping; Wang, Xiaobin S et al. (2018) Kinase-dependent structural role of DNA-PKcs during immunoglobulin class switch recombination. Proc Natl Acad Sci U S A 115:8615-8620
Gnügge, Robert; Symington, Lorraine S (2017) Keeping it real: MRX-Sae2 clipping of natural substrates. Genes Dev 31:2311-2312
Liu, Xiangyu; Shao, Zhengping; Jiang, Wenxia et al. (2017) PAXX promotes KU accumulation at DNA breaks and is essential for end-joining in XLF-deficient mice. Nat Commun 8:13816
Kato, Niyo; Kawasoe, Yoshitaka; Williams, Hannah et al. (2017) Sensing and Processing of DNA Interstrand Crosslinks by the Mismatch Repair Pathway. Cell Rep 21:1375-1385
Aparicio, Tomas; Gautier, Jean (2016) BRCA1-CtIP interaction in the repair of DNA double-strand breaks. Mol Cell Oncol 3:e1169343

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