Abstract

Many laboratories are making mutations in the serotonin, norepinephrine, and dopamine transporters (SERT, NET and DAT, respectively) and in related transporters in the NaCl-coupled transporter family. The properties of these transporter mutants are published in many scientific articles scattered widely through the literature. This core will create a database of all published mutations in any member of this transporter family. The mutations will be organized according to position, family member and the effect on transporter function. The results will be presented in a World Wide Web site so that anyone can access the information easily. We expect that this will facilitate the rapid sharing of information in this field, and will benefit many drug abuse researchers. As the structural modeling efforts become more refined by the experimental results from this program, we intend also to display the current structural model for the transporter family. A three dimensional representation of this model will be available for visitors to the website to tilt and rotate using VRML (Virtual Reality Markup Language).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Program Projects (P01)
Project #
5P01DA012408-04
Application #
6618887
Study Section
Project Start
2002-07-01
Project End
2003-06-30
Budget Start
Budget End
Support Year
4
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Mount Sinai School of Medicine
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029

  Publications

Mayer, Felix P; Schmid, Diethart; Owens, W Anthony et al. (2018) An unsuspected role for organic cation transporter 3 in the actions of amphetamine. Neuropsychopharmacology 43:2408-2417
Quick, Matthias; Abramyan, Ara M; Wiriyasermkul, Pattama et al. (2018) The LeuT-fold neurotransmitter:sodium symporter MhsT has two substrate sites. Proc Natl Acad Sci U S A 115:E7924-E7931
Herborg, Freja; Andreassen, Thorvald F; Berlin, Frida et al. (2018) Neuropsychiatric disease-associated genetic variants of the dopamine transporter display heterogeneous molecular phenotypes. J Biol Chem 293:7250-7262
Razavi, Asghar M; Khelashvili, George; Weinstein, Harel (2018) How structural elements evolving from bacterial to human SLC6 transporters enabled new functional properties. BMC Biol 16:31
Doktorova, Milka; Weinstein, Harel (2018) Accurate In Silico Modeling of Asymmetric Bilayers Based on Biophysical Principles. Biophys J 115:1638-1643
LeVine, Michael V; Cuendet, Michel A; Razavi, Asghar M et al. (2018) Thermodynamic Coupling Function Analysis of Allosteric Mechanisms in the Human Dopamine Transporter. Biophys J 114:10-14
Wragg, Rachel T; Parisotto, Daniel A; Li, Zhenlong et al. (2017) Evolutionary Divergence of the C-terminal Domain of Complexin Accounts for Functional Disparities between Vertebrate and Invertebrate Complexins. Front Mol Neurosci 10:146
Doktorova, M; Harries, D; Khelashvili, G (2017) Determination of bending rigidity and tilt modulus of lipid membranes from real-space fluctuation analysis of molecular dynamics simulations. Phys Chem Chem Phys 19:16806-16818
Runegaard, Annika H; Jensen, Kathrine L; Fitzpatrick, Ciarán M et al. (2017) Preserved dopaminergic homeostasis and dopamine-related behaviour in hemizygous TH-Cre mice. Eur J Neurosci 45:121-128
Jensen, Kathrine L; Runegaard, Annika H; Weikop, Pia et al. (2017) Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake. J Vis Exp :

Showing the most recent 10 out of 146 publications