Abstract

Core C: Flow Cytometry Core. The Flow Cytometry Core will provide services to each of the Projects in this Program Project grant. The facility will perform multi-color cell surface antigen detection analysis that is required for the immunophenotyping of leukocytes. In addition, analysis of intracellular markers that are required for various immunological analyses will be performed, including analysis of apoptosis. The Core will also provide biomarker analysis, which will be carried out by cytometric bead array technology. The Core is well equiped for cell sorting of primary cells, as well as transfected cell lines. This incudes the capacity to sort individual cells for the purpose of cell cloning. Finally, the Core will provide a complete survey of consultation and advice in the proper design of experiments which utilize flow cytometry.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Program Projects (P01)
Project #
5P01DA023860-05
Application #
8378174
Study Section
Special Emphasis Panel (ZDA1-RXL-E)
Project Start
Project End
2014-04-30
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
5
Fiscal Year
2012
Total Cost
$87,885
Indirect Cost
$27,676

  Institution

Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122

  Publications

Regan, Patrick M; Langford, Dianne; Khalili, Kamel (2016) Regulation and Functional Implications of Opioid Receptor Splicing in Opioid Pharmacology and HIV Pathogenesis. J Cell Physiol 231:976-85
Gabunia, Khatuna; Ellison, Stephen; Kelemen, Sheri et al. (2016) IL-19 Halts Progression of Atherosclerotic Plaque, Polarizes, and Increases Cholesterol Uptake and Efflux in Macrophages. Am J Pathol 186:1361-74
Cornwell, William D; Wagner, Wendeline; Lewis, Mark G et al. (2016) Effect of chronic morphine administration on circulating dendritic cells in SIV-infected rhesus macaques. J Neuroimmunol 295-296:30-40
Song, Changcheng; Wang, Qing; Song, Changzheng et al. (2015) Nucleocytoplasmic shuttling of valosin-containing protein (VCP/p97) regulated by its N domain and C-terminal region. Biochim Biophys Acta 1853:222-32
Cabral, Guy A; Rogers, Thomas J; Lichtman, Aron H (2015) Turning Over a New Leaf: Cannabinoid and Endocannabinoid Modulation of Immune Function. J Neuroimmune Pharmacol 10:193-203
Pitcher, Jonathan; Abt, Anna; Myers, Jaclyn et al. (2014) Neuronal ferritin heavy chain and drug abuse affect HIV-associated cognitive dysfunction. J Clin Invest 124:656-69
Bednar, Filip; Song, Changcheng; Bardi, Giuseppe et al. (2014) Cross-desensitization of CCR1, but not CCR2, following activation of the formyl peptide receptor FPR1. J Immunol 192:5305-13
Cornwell, William D; Lewis, Mark G; Fan, Xiaoxuan et al. (2013) Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques. J Neuroimmunol 265:43-50
Dave, Rajnish S (2012) Morphine affects HIV-induced inflammatory response without influencing viral replication in human monocyte-derived macrophages. FEMS Immunol Med Microbiol 64:228-36
Rogers, Thomas J (2012) The molecular basis for neuroimmune receptor signaling. J Neuroimmune Pharmacol 7:722-4

Showing the most recent 10 out of 18 publications