The Image Analysis and Quantitative Morphometry Core provides Program Project Investigators a wide variety of options for the quantitative analysis from histological preparations. The Core Resource has: a Zeiss LSM (laser scanning microscope) confocal microscope; several research microscopes interfaced for photography, digital image capture and computer driven stage control; image digitization from microscopes photographs and drawings; CD writing for archival storage; up to date generate , special purpose and locally modified commercial and public domain software; and network connections to all investigators The resources evaluates: regional areas and volumes; projection (whisker like) patterns; neuron number/phenotype; relative message and protein density; cell death; and process length, branching and target size. Materials and images are evaluated directly by expert Core morphologists (blinded to experimental conditions when necessary) and analyzed by appropriate statistics in consultation with the Core statistician. The Core contributes intellectually in preparation of materials for presentation and publication and makes illustrations for manuscripts and 35 mm sides. The Core plans to modernize some of its special purpose hardware and software. The Core streamlines data collection by writing macros. The Core will facilitate Program Project communication via its web page. This Core contributes to the general theme of this Program Project which is directed to understanding mechanisms of developmental plasticity as a basis for rational therapy of conditions affecting the nervous system, especially as they relate to orofacial sensation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Program Projects (P01)
Project #
5P01DE007734-16
Application #
6451077
Study Section
Special Emphasis Panel (ZDE1)
Project Start
1989-12-01
Project End
2005-03-31
Budget Start
Budget End
Support Year
16
Fiscal Year
2001
Total Cost
Indirect Cost
Name
Washington University
Department
Type
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Jacquin, Mark F; Arends, Joop J A; Renehan, William E et al. (2015) Whisker-related circuitry in the trigeminal nucleus principalis: Topographic precision. Somatosens Mot Res 32:8-20
Xiang, Chuanxi; Arends, Joop J A; Jacquin, Mark F (2014) Whisker-related circuitry in the trigeminal nucleus principalis: ultrastructure. Somatosens Mot Res 31:141-51
Vadivelu, Sudhakar; Platik, Marina M; Choi, Luke et al. (2005) Multi-germ layer lineage central nervous system repair: nerve and vascular cell generation by embryonic stem cells transplanted in the injured brain. J Neurosurg 103:124-35
Pluto, Charles P; Chiaia, Nicolas L; Rhoades, Robert W et al. (2005) Reducing contralateral SI activity reveals hindlimb receptive fields in the SI forelimb-stump representation of neonatally amputated rats. J Neurophysiol 94:1727-32
Genc, Baris; Ulupinar, Emel; Erzurumlu, Reha S (2005) Differential Trk expression in explant and dissociated trigeminal ganglion cell cultures. J Neurobiol 64:145-56
Gandhi, Rohan; Ryals, Janelle M; Wright, Douglas E (2004) Neurotrophin-3 reverses chronic mechanical hyperalgesia induced by intramuscular acid injection. J Neurosci 24:9405-13
McDonald, John W; Becker, Daniel; Holekamp, Terrence F et al. (2004) Repair of the injured spinal cord and the potential of embryonic stem cell transplantation. J Neurotrauma 21:383-93
Genc, Baris; Ozdinler, P Hande; Mendoza, April E et al. (2004) A chemoattractant role for NT-3 in proprioceptive axon guidance. PLoS Biol 2:e403
Ulupinar, Emel; Unal, Nedim; Erzurumlu, Reha S (2004) Morphometric analysis of embryonic rat trigeminal neurons treated with different neurotrophins. Anat Rec A Discov Mol Cell Evol Biol 277:396-407
Wright, Douglas E; Ryals, Janelle M; McCarson, Kenneth E et al. (2004) Diabetes-induced expression of activating transcription factor 3 in mouse primary sensory neurons. J Peripher Nerv Syst 9:242-54

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