Abstract

The objectives of this research are to determine the basic mechanisms that regulate the interactions between cytoskeletal keratin intermediate filaments and the cell surface associated adhesion junctions, desmosomes and hemidesmosomes, of gingival epithelia cells. The long term goal of the proposed studies is to gain insights into the molecular bases of the epithelial cell migration that represents a hallmark of periodontal disease.
The specific aims i nclude determining how the intermediate filament associated protein, IFAP300, connects desmosomes and hemidesmosomes to intermediate filaments. To this end, IFAP300 will be cloned and sequenced. Once the complete cDNA sequence is available, transient transfection will be used to determine the physiological functions of this important cross-bridging protein.
Other aims are to determine in vitro the molecular interactions among keratins, IFAP300 and the major cytoplasmic plaque proteins of the desmosome (desmoplakin) and the hemidesmosome (BP230). In vivo studies will be targeted at determining the dynamic properties of keratin-intermediate filaments in living cells using microscopic imaging techniques.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Program Projects (P01)
Project #
5P01DE012328-05
Application #
6448507
Study Section
Project Start
1997-08-01
Project End
2002-05-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
2001
Total Cost
$129,435
Indirect Cost

  Institution

Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611

  Publications

Godsel, Lisa M; Hobbs, Ryan P; Green, Kathleen J (2008) Intermediate filament assembly: dynamics to disease. Trends Cell Biol 18:28-37
Dusek, Rachel L; Godsel, Lisa M; Green, Kathleen J (2007) Discriminating roles of desmosomal cadherins: beyond desmosomal adhesion. J Dermatol Sci 45:7-21
Green, Kathleen J; Simpson, Cory L (2007) Desmosomes: new perspectives on a classic. J Invest Dermatol 127:2499-515
Dusek, Rachel L; Getsios, Spiro; Chen, Feng et al. (2006) The differentiation-dependent desmosomal cadherin desmoglein 1 is a novel caspase-3 target that regulates apoptosis in keratinocytes. J Biol Chem 281:3614-24
Ghosh, Supurna; Johnson, Jeff J; Sen, Ratna et al. (2006) Functional relevance of urinary-type plasminogen activator receptor-alpha3beta1 integrin association in proteinase regulatory pathways. J Biol Chem 281:13021-9
Munshi, H G; Stack, M S (2006) Reciprocal interactions between adhesion receptor signaling and MMP regulation. Cancer Metastasis Rev 25:45-56
Natarajan, Easwar; Omobono 2nd, John D; Guo, Zongyou et al. (2006) A keratinocyte hypermotility/growth-arrest response involving laminin 5 and p16INK4A activated in wound healing and senescence. Am J Pathol 168:1821-37
Godsel, Lisa M; Hsieh, Sherry N; Amargo, Evangeline V et al. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol 171:1045-59
Yin, Taofei; Getsios, Spiro; Caldelari, Reto et al. (2005) Plakoglobin suppresses keratinocyte motility through both cell-cell adhesion-dependent and -independent mechanisms. Proc Natl Acad Sci U S A 102:5420-5
Green, Kathleen J; Bohringer, Michael; Gocken, Todd et al. (2005) Intermediate filament associated proteins. Adv Protein Chem 70:143-202

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