Abstract

The overall hypothesis to be tested is that Twist is involved in negative regulation of myogenesis by interfering with required transcriptional coregulators, in particular p300 and/or PCAF, and by interacting with other regulators of early myogenic differentiation, such as Msx. This hypothesis will be tested by molecular genetic as well as biochemical analysis of a) mechanisms by which mouse Twist influences the transcription activation, by MEF2 and bHLH proteins of the different steps that eventually lead to the myogenic phenotype; b) the role of p300 and PCAF in this process; c) the interaction between Twist and Msx proteins; and d) a search for the existence and role of muscle restricted partners of p300, P/CAF and Twist. The isolation of co-regulators of muscle regulatory genes and understanding the roles of these molecules will be valuable toward our eventual goal, the development of preventive and therapeutic approaches to early diagnosis and intervention of craniofacial malformation and dygenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Program Projects (P01)
Project #
5P01DE012941-03
Application #
6480416
Study Section
Project Start
1999-08-01
Project End
2004-07-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2001
Total Cost
$130,674
Indirect Cost

  Institution

Name
University of Southern California
Department
Type
DUNS #
041544081
City
Los Angeles
State
CA
Country
United States
Zip Code
90089

  Publications

Nakajima, Akira; Ito, Yoshihiro; Tanaka, Eiji et al. (2014) Functional role of TGF-? receptors during palatal fusion in vitro. Arch Oral Biol 59:1192-204
Nakajima, Akira; Tanaka, Eiji; Ito, Yoshihiro et al. (2010) The expression of TGF-?3 for epithelial-mesenchyme transdifferentiated MEE in palatogenesis. J Mol Histol 41:343-55
Ting, Man-Chun; Wu, Nancy L; Roybal, Paul G et al. (2009) EphA4 as an effector of Twist1 in the guidance of osteogenic precursor cells during calvarial bone growth and in craniosynostosis. Development 136:855-64
Zhuang, Fengfeng; Nguyen, Manuel P; Shuler, Charles et al. (2009) Analysis of Msx1 and Msx2 transactivation function in the context of the heat shock 70 (Hspa1b) gene promoter. Biochem Biophys Res Commun 381:241-6
Zhuang, Fengfeng; Yen, Philip; Zhao, Jiangyue et al. (2008) Dynamic intracellular distribution of Eaf2 and its potential involvement in UV-Induced DNA damage response. DNA Cell Biol 27:649-56
Maxson, Robert; Ishii, Mamoru (2008) The Bmp pathway in skull vault development. Front Oral Biol 12:197-208
Fu, Hualin; Ishii, Mamoru; Gu, Ying et al. (2007) Conditional alleles of Msx1 and Msx2. Genesis 45:477-81
Nakajima, Akira; Ito, Yoshihiro; Asano, Masatake et al. (2007) Functional role of transforming growth factor-beta type III receptor during palatal fusion. Dev Dyn 236:791-801
Chen, Yi-Hui; Ishii, Mamoru; Sun, Jingjing et al. (2007) Msx1 and Msx2 regulate survival of secondary heart field precursors and post-migratory proliferation of cardiac neural crest in the outflow tract. Dev Biol 308:421-37
Kong, Kimi Y; Kedes, Larry (2006) Leucine 135 of tropomodulin-1 regulates its association with tropomyosin, its cellular localization, and the integrity of sarcomeres. J Biol Chem 281:9589-99

Showing the most recent 10 out of 36 publications