Abstract

The Biological Core will be responsible for extracting and purifying fimbriae, LPS and lipid A from Porphyromonas gingivalis. Standard biochemical techniques will be employed for these procedures. The core will also construct the P. gingivalis fimA deletion mutant. In addition, the core will coordinate the commercial production of polyclonal and monoclonal antibodies to P. gingivalis fimbriae, to the endothelial receptor, to Toll-like proteins and moesin. Finally, the biological core will coordinate sample preparation for nucleotide and peptide sequencing and flow cytometry.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Program Projects (P01)
Project #
5P01DE013191-02
Application #
6438580
Study Section
Special Emphasis Panel (ZDE1)
Project Start
2000-04-01
Project End
2005-03-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
$211,997
Indirect Cost

  Institution

Name
Boston Medical Center
Department
Type
DUNS #
005492160
City
Boston
State
MA
Country
United States
Zip Code
02118

  Publications

Janket, S; Meurman, J H; Baird, A E et al. (2010) Salivary immunoglobulins and prevalent coronary artery disease. J Dent Res 89:389-94
Qvarnstrom, M; Janket, S; Jones, J A et al. (2008) Salivary lysozyme and prevalent hypertension. J Dent Res 87:480-4
Janket, Sok-Ja; Jones, Judith; Rich, Sharron et al. (2007) The effects of xerogenic medications on oral mucosa among the Veterans Dental Study participants. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 103:223-30
Miyamoto, Takanari; Yumoto, Hiromichi; Takahashi, Yusuke et al. (2006) Pathogen-accelerated atherosclerosis occurs early after exposure and can be prevented via immunization. Infect Immun 74:1376-80
Takahashi, Yusuke; Davey, Michael; Yumoto, Hiromichi et al. (2006) Fimbria-dependent activation of pro-inflammatory molecules in Porphyromonas gingivalis infected human aortic endothelial cells. Cell Microbiol 8:738-57
Zhou, Qingde; Desta, Tesfahun; Fenton, Matthew et al. (2005) Cytokine profiling of macrophages exposed to Porphyromonas gingivalis, its lipopolysaccharide, or its FimA protein. Infect Immun 73:935-43
Gyurko, R; Shoji, H; Battaglino, R A et al. (2005) Inducible nitric oxide synthase mediates bone development and P. gingivalis-induced alveolar bone loss. Bone 36:472-9
Gibson 3rd, Frank C; Savelli, Juan; Van Dyke, Thomas E et al. (2005) Gingipain-specific IgG in the sera of patients with periodontal disease is necessary for opsonophagocytosis of Porphyromonas gingivalis. J Periodontol 76:1629-36
Chou, Hsin-Hua; Yumoto, Hiromichi; Davey, Michael et al. (2005) Porphyromonas gingivalis fimbria-dependent activation of inflammatory genes in human aortic endothelial cells. Infect Immun 73:5367-78
Graves, D T; Naguib, G; Lu, H et al. (2005) Inflammation is more persistent in type 1 diabetic mice. J Dent Res 84:324-8

Showing the most recent 10 out of 30 publications