Abstract

Enamelin is a major extracellular component of dental enamel, yet its role in the formation of this tissue has not been extensively explored.
Specific aims of this project are: 1) to determine the temporal and spatial expression of enamelin during the secretory, transition, and maturation stages of enamel biomineralization; 2) to generate a enamelin knockout mouse and characterized enamel formation in the absence of enamelin expression; and 3) to investigate the regulation of mouse enamelin gene expression. Planned approaches to accomplish these aims are to examine the temporal and spacial distribution of enamelin gene expression using immunohistochemistry and in situ hybridization, to knock out the enamelin gene and examine ensuing changes in enamel formation, and to utilize systematic deletion/mutation analysis to identify regions of the enamelin gene necessary for cell and stage-specific expression in ameloblasts. Information gained from these studies taken together with findings related to the transcriptional regulation of other proteins such as amelogenin, ameloblastin, EMSP1 and DSPP will allow a comprehensive understanding of the regulation of structural gene expression during amelogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Program Projects (P01)
Project #
5P01DE013221-02
Application #
6452275
Study Section
Special Emphasis Panel (ZDE1)
Project Start
2000-04-01
Project End
2005-03-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2001
Total Cost
$126,832
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229

  Publications

Chen, S; Gluhak-Heinrich, J; Wang, Y H et al. (2009) Runx2, osx, and dspp in tooth development. J Dent Res 88:904-9
Hu, Yuanyuan; Papagerakis, Petros; Ye, Ling et al. (2008) Distal cis-regulatory elements are required for tissue-specific expression of enamelin (Enam). Eur J Oral Sci 116:113-23
Chen, Shuo; Chen, Lei; Jahangiri, Allen et al. (2008) Expression and processing of small integrin-binding ligand N-linked glycoproteins in mouse odontoblastic cells. Arch Oral Biol 53:879-89
Wittrant, Y; Bhandari, B Sriniketan; Abboud, H et al. (2008) PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells. J Dent Res 87:33-8
Werner, S Abboud; Gluhak-Heinrich, J; Woodruff, K et al. (2007) Targeted expression of csCSF-1 in op/op mice ameliorates tooth defects. Arch Oral Biol 52:432-43
Paine, Michael L; Luo, Wen; Wang, Hong-Jun et al. (2005) Dentin sialoprotein and dentin phosphoprotein overexpression during amelogenesis. J Biol Chem 280:31991-8
Tu, Qisheng; Yamauchi, Masato; Pageau, Steven C et al. (2004) Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells. Biochem Biophys Res Commun 316:461-7
Ye, Ling; MacDougall, Mary; Zhang, Shubin et al. (2004) Deletion of dentin matrix protein-1 leads to a partial failure of maturation of predentin into dentin, hypomineralization, and expanded cavities of pulp and root canal during postnatal tooth development. J Biol Chem 279:19141-8
Zhang, J H; Wang, J; Tang, J et al. (2004) Bone sialoprotein promotes bone metastasis of a non-bone-seeking clone of human breast cancer cells. Anticancer Res 24:1361-8
Zhang, Jian-Hua; Tang, Jean; Wang, Jie et al. (2003) Over-expression of bone sialoprotein enhances bone metastasis of human breast cancer cells in a mouse model. Int J Oncol 23:1043-8

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