Abstract

The mouse core will support the various components of the program in two major ways: a) the preparation of transgenic and gene knockout mice; and, b) the maintenance of various mutant mouse lines to be used in the proposed studies. A major initial focus of the core will relate to the creation of both protein 4.1 knockout mice and mice expressing various protein 4.1 transgenes. The proposed studies to dissect in vivo the biological functions of protein 4.1 are described in projects 1, 2, 3, and 6. The core will play a central role in assisting the participating programs in designing the various aspects involved in of the murine studies. All the technical steps in creating the transgenic and gene knock- out mice (i.e. creating the various DNA constructs and performing embryo manipulations) will be carried out as part of this core. The core will also maintain and provide thalassemic mice.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
2P01DK032094-15
Application #
6270558
Study Section
Project Start
1997-12-20
Project End
1998-11-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
15
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Lawrence Berkeley National Laboratory
Department
Type
DUNS #
078576738
City
Berkeley
State
CA
Country
United States
Zip Code
94720

  Publications

Qu, Xiaoli; Zhang, Shijie; Wang, Shihui et al. (2018) TET2 deficiency leads to stem cell factor-dependent clonal expansion of dysfunctional erythroid progenitors. Blood 132:2406-2417
Huang, Yumin; Hale, John; Wang, Yaomei et al. (2018) SF3B1 deficiency impairs human erythropoiesis via activation of p53 pathway: implications for understanding of ineffective erythropoiesis in MDS. J Hematol Oncol 11:19
Ali, Abdullah Mahmood; Huang, Yumin; Pinheiro, Ronald Feitosa et al. (2018) Severely impaired terminal erythroid differentiation as an independent prognostic marker in myelodysplastic syndromes. Blood Adv 2:1393-1402
Yan, Hongxia; Hale, John; Jaffray, Julie et al. (2018) Developmental differences between neonatal and adult human erythropoiesis. Am J Hematol 93:494-503
Han, Xu; Zhang, Jieying; Peng, Yuanliang et al. (2017) Unexpected role for p19INK4d in posttranscriptional regulation of GATA1 and modulation of human terminal erythropoiesis. Blood 129:226-237
Gastou, Marc; Rio, Sarah; Dussiot, Michaël et al. (2017) The severe phenotype of Diamond-Blackfan anemia is modulated by heat shock protein 70. Blood Adv 1:1959-1976
Irianto, Jerome; Pfeifer, Charlotte R; Xia, Yuntao et al. (2016) SnapShot: Mechanosensing Matrix. Cell 165:1820-1820.e1
Pimentel, Harold; Parra, Marilyn; Gee, Sherry L et al. (2016) A dynamic intron retention program enriched in RNA processing genes regulates gene expression during terminal erythropoiesis. Nucleic Acids Res 44:838-51
Dingal, P C Dave P; Bradshaw, Andrew M; Cho, Sangkyun et al. (2015) Fractal heterogeneity in minimal matrix models of scars modulates stiff-niche stem-cell responses via nuclear exit of a mechanorepressor. Nat Mater 14:951-60
Blanc, Lionel; Papoin, Julien; Debnath, Gargi et al. (2015) Abnormal erythroid maturation leads to microcytic anemia in the TSAP6/Steap3 null mouse model. Am J Hematol 90:235-41

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