Abstract

The Laboratory Core will support the program project investigating the clinical and cellular aspects of non-insulin dependent diabetes mellitus (NIDDM), including research projects investigating mechanisms of insulin resistance in liver, muscle, and adipose tissue from NIDDM and obese humans and lipoprotein metabolism. Specifically, the Laboratory Core will direct, organize, coordinate, and perform anatomic and clinical chemistry analysis of all the research projects. Light microscopic assessment of liver and muscle tissue submitted at the time of gastric bypass surgery for morbid obesity will be performed along with electron microscopic examination of liver, muscle and cultured tissue cells. Muscle histochemistry and immunohistochemistry examination on liver tissue will complement the histologic and electron microscopic evaluation. Clinical chemistry studies including radioimmunoassay and other laboratory analysis will support all four projects. These morphologic and clinical chemistry analyses will support research projects hoping to bridge the gap of cellular research and clinical investigation into the mechanisms of insulin resistance seen in type II diabetes mellitus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK036296-03
Application #
3897470
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
East Carolina University
Department
Type
DUNS #
City
Greenville
State
NC
Country
United States
Zip Code
27858

  Publications

Friedman, J E; Caro, J F; Pories, W J et al. (1994) Glucose metabolism in incubated human muscle: effect of obesity and non-insulin-dependent diabetes mellitus. Metabolism 43:1047-54
Long, S D; O'Brien, K; MacDonald Jr, K G et al. (1994) Weight loss in severely obese subjects prevents the progression of impaired glucose tolerance to type II diabetes. A longitudinal interventional study. Diabetes Care 17:372-5
Elton, C W; Tapscott, E B; Pories, W J et al. (1994) Effect of moderate obesity on glucose transport in human muscle. Horm Metab Res 26:181-3
Houmard, J A; Shinebarger, M H; Dolan, P L et al. (1993) Exercise training increases GLUT-4 protein concentration in previously sedentary middle-aged men. Am J Physiol 264:E896-901
Houmard, J A; Hortobagyi, T; Neufer, P D et al. (1993) Training cessation does not alter GLUT-4 protein levels in human skeletal muscle. J Appl Physiol 74:776-81
Spiegelman, D; Israel, R G; Bouchard, C et al. (1992) Absolute fat mass, percent body fat, and body-fat distribution: which is the real determinant of blood pressure and serum glucose? Am J Clin Nutr 55:1033-44
Friedman, J E; Dohm, G L; Leggett-Frazier, N et al. (1992) Restoration of insulin responsiveness in skeletal muscle of morbidly obese patients after weight loss. Effect on muscle glucose transport and glucose transporter GLUT4. J Clin Invest 89:701-5
Caro, J F; Jenquin, M; Long, S (1992) Effects of phorbol esters on insulin receptor function and insulin action in hepatocytes: evidence for heterogeneity. Mol Cell Biochem 109:115-8
Contreras, I; Caro, J F; Aveledo, L et al. (1992) In chronic uremia, insulin activates receptor kinase but not pyruvate dehydrogenase. Nephron 61:77-81
Pories, W J; MacDonald Jr, K G; Flickinger, E G et al. (1992) Is type II diabetes mellitus (NIDDM) a surgical disease? Ann Surg 215:633-42;discussion 643

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