Abstract

The pancreatic islet isolation and vector core (Core B) is an integral component of this program project. The purpose of this core is to assist the project leaders and their investigative personnel in three critical ways: 1) isolation of large numbers of highly purified pancreatic islets, 2) quality control of purity, quantity, and viability of islets needed for physiological and molecular studies as well as for successful transplantation in diabetic recipients and 3) construction and quality controlled delivery of adenoviral vectors containing the desired recombinant gene. The overlapping and synergistic need for both isolated islet and recombinant adenoviral vectors by the investigators of this program project prompted us to propose the implementation of Core B. The need for such a core is particularly poignant given the lack of a bona fide and easily accessible islet isolation core at the University of Pennsylvania. Additionally, given our 'in-house' expertise in construction of adenoviral vectors, we fell that the most time- and cost-efficient means of developing adenoviral vectors is the proposed 'in house' Core facility (Core B).

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK049814-08
Application #
6609130
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-06-01
Project End
2003-05-31
Budget Start
Budget End
Support Year
8
Fiscal Year
2002
Total Cost
$186,513
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Sun, Zheng; Miller, Russell A; Patel, Rajesh T et al. (2012) Hepatic Hdac3 promotes gluconeogenesis by repressing lipid synthesis and sequestration. Nat Med 18:934-42
Zhao, G; Moore, D J; Lee, K M et al. (2010) An unexpected counter-regulatory role of IL-10 in B-lymphocyte-mediated transplantation tolerance. Am J Transplant 10:796-801
Yang, Jichun; Wang, Chunjiong; Li, Jing et al. (2009) PANDER binds to the liver cell membrane and inhibits insulin signaling in HepG2 cells. FEBS Lett 583:3009-15
Huang, Xiaolun; Moore, Daniel J; Mohiuddin, Mohammad et al. (2008) Inhibition of ICAM-1/LFA-1 interactions prevents B-cell-dependent anti-CD45RB-induced transplantation tolerance. Transplantation 85:675-80
Noorchashm, Hooman; Reed, Amy J; Rostami, Susan Y et al. (2006) B cell-mediated antigen presentation is required for the pathogenesis of acute cardiac allograft rejection. J Immunol 177:7715-22
Quinn 3rd, William J; Noorchashm, Negin; Crowley, Jenni E et al. (2006) Cutting edge: impaired transitional B cell production and selection in the nonobese diabetic mouse. J Immunol 176:7159-64
Yang, Jichun; Wong, Ryan K; Park, MieJung et al. (2006) Leucine regulation of glucokinase and ATP synthase sensitizes glucose-induced insulin secretion in pancreatic beta-cells. Diabetes 55:193-201
Burkhardt, Brant R; Greene, Scott R; White, Peter et al. (2006) PANDER-induced cell-death genetic networks in islets reveal central role for caspase-3 and cyclin-dependent kinase inhibitor 1A (p21). Gene 369:134-41
Yang, Jichun; Gao, Zhiyong; Robert, Claudia E et al. (2005) Structure-function studies of PANDER, an islet specific cytokine inducing cell death of insulin-secreting beta cells. Biochemistry 44:11342-52
Reed, Amy J; Zarrabi, Yasaman; Perate, Alison L et al. (2005) The frequency of double-positive thymocytes expressing an alphabeta TCR clonotype regulates peripheral CD4 T cell compartment homeostasis. Immunology 116:400-7

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