Abstract

We plan to use a powerful new technology, massively parallel signature sequencing, to determine the complete transcriptomes (all mRNAs) of four hematopoietic stem cell populations, long-term hematopoietic stem cells (LT-HSC), short-term hematopoietic stem cells (ST-HSC), common lymphoid progenitor (CLP), and common myeloid progenitor (CMP), as well as the model hematopoietic cell line (EML-C1). We will place all of these identified genes (mRNAs) on DNA microarrays and oligonucleotide arrays to interrogate the gene expression patterns of selected hematopoietic stem cell populations. For example, we will employ gene knockout experiments to perturb an important signal transduction pathway in hematopoietic development, Wnt-beta-catenin, ion a cell line (EML-C1) using systems approaches. We will also use quantitative methods of proteomics to explore the protein expression patterns in hematopoietic stem cells and a progenitor cell line (EML-C1) using systems approaches. We will also use quantitative methods of proteomics to explore the protein expression patterns in hematopoietic stem cells and a progenitor cell line (EML-C1). We will identify orthologous and paralogous relationships between murine and Drosophila genes involved in stem cell biology. From these analyses will come a heightened understanding of the genes and informational pathways involved with murine hematopoietic stem cell self-renewal and development.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
2P01DK053074-06
Application #
6583557
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-09-01
Project End
2007-08-31
Budget Start
Budget End
Support Year
6
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Sun, Bingyun; Ma, Li; Yan, Xiaowei et al. (2013) N-glycoproteome of E14.Tg2a mouse embryonic stem cells. PLoS One 8:e55722
Bockhorn, Jessica; Yee, Kathy; Chang, Ya-Fang et al. (2013) MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion. Breast Cancer Res Treat 137:373-82
Heffner, Garrett C; Clutter, Matthew R; Nolan, Garry P et al. (2011) Novel hematopoietic progenitor populations revealed by direct assessment of GATA1 protein expression and cMPL signaling events. Stem Cells 29:1774-82
Yan, Xiaowei; Ma, Li; Yi, Danielle et al. (2011) A CD133-related gene expression signature identifies an aggressive glioblastoma subtype with excessive mutations. Proc Natl Acad Sci U S A 108:1591-6
Forsberg, E Camilla; Passegué, Emmanuelle; Prohaska, Susan S et al. (2010) Molecular signatures of quiescent, mobilized and leukemia-initiating hematopoietic stem cells. PLoS One 5:e8785
Jaiswal, Siddhartha; Chao, Mark P; Majeti, Ravindra et al. (2010) Macrophages as mediators of tumor immunosurveillance. Trends Immunol 31:212-9
Inaba, Mayu; Yuan, Hebao; Salzmann, Viktoria et al. (2010) E-cadherin is required for centrosome and spindle orientation in Drosophila male germline stem cells. PLoS One 5:e12473
Papathanasiou, Peter; Attema, Joanne L; Karsunky, Holger et al. (2009) Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150. Stem Cells 27:2498-508
Majeti, Ravindra; Becker, Michael W; Tian, Qiang et al. (2009) Dysregulated gene expression networks in human acute myelogenous leukemia stem cells. Proc Natl Acad Sci U S A 106:3396-401
Jaiswal, Siddhartha; Jamieson, Catriona H M; Pang, Wendy W et al. (2009) CD47 is upregulated on circulating hematopoietic stem cells and leukemia cells to avoid phagocytosis. Cell 138:271-85

Showing the most recent 10 out of 38 publications