Abstract

application) This core will provide a basis for shared reagents and analytical technologies for the three projects. The core will incorporate a series of common analytical techniques. It will measure iron and related metals in biological fluids using atomic absorption spectroscopy. It will develop oligonucleotide and molecular genetic probes needed for accurate and sensitive analysis of iron-related regulatory proteins. It will also standardize common antibodies to be used in the projects to measure iron-related proteins. The overall goal of the core is to serve as a point for technologic and intellectual synergy between projects and create facilities in which iron and other metals, and proteins can be measured with sensitivity, accuracy, and reproducibility.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
3P01DK053430-04S1
Application #
6662818
Study Section
Project Start
2002-01-01
Project End
2002-12-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
4
Fiscal Year
2002
Total Cost
$140,000
Indirect Cost

  Institution

Name
Pennsylvania State University
Department
Type
DUNS #
129348186
City
Hershey
State
PA
Country
United States
Zip Code
17033

  Publications

Patton, Stephanie M; Pinero, Domingo J; Surguladze, Nodar et al. (2005) Subcellular localization of iron regulatory proteins to Golgi and ER membranes. J Cell Sci 118:4365-73
Henderson, Rebecca J; Patton, Stephanie M; Connor, James R (2005) Development of a fluorescent reporter to assess iron regulatory protein activity in living cells. Biochim Biophys Acta 1743:162-8
Grundy, Martin A; Gorman, Nadia; Sinclair, Peter R et al. (2004) High-throughput non-heme iron assay for animal tissues. J Biochem Biophys Methods 59:195-200
Bilello, John P; Cable, Edward E; Isom, Harriet C (2003) Expression of E-cadherin and other paracellular junction genes is decreased in iron-loaded hepatocytes. Am J Pathol 162:1323-38
Bilello, J P; Cable, E E; Myers, R L et al. (2003) Role of paracellular junction complexes in baculovirus-mediated gene transfer to nondividing rat hepatocytes. Gene Ther 10:733-49
Iocca, Heather A; Isom, Harriet C (2003) Tumor necrosis factor-alpha acts as a complete mitogen for primary rat hepatocytes. Am J Pathol 163:465-76
Stoehr, Stephanie A; Isom, Harriet C (2003) Gap junction-mediated intercellular communication in a long-term primary mouse hepatocyte culture system. Hepatology 38:1125-35
Chorney, Michael J; Yoshida, Yukinori; Meyer, Paul N et al. (2003) The enigmatic role of the hemochromatosis protein (HFE) in iron absorption. Trends Mol Med 9:118-25
Malecki, Elise A; Cable, Edward E; Isom, Harriet C et al. (2002) The lipophilic iron compound TMH-ferrocene [(3,5,5-trimethylhexanoyl)ferrocene] increases iron concentrations, neuronal L-ferritin, and heme oxygenase in brains of BALB/c mice. Biol Trace Elem Res 86:73-84
Gerhard, Glenn S; Kaufmann, Elizabeth J; Wang, Xujun et al. (2002) Genetic differences in hepatic lipid peroxidation potential and iron levels in mice. Mech Ageing Dev 123:167-76

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