Abstract

This is a core facility designed to (1) establish cell lines and extract nucleic acids from clinical material and (2) perform molecular genotype analysis. The core will support Project 3 (Mapping of the Cyclic Hematopoiesis Gene) and Project 4( Mapping of the Familial Leukemia Gene). For Project 3, this unit will process venous blood and bone marrow specimens and will be responsible for additional genotype analysis of the region first identified in family 619. For Project 4, this core facility will prepare immortalized lymphoblastoid cell lines from members of leukemia families and store aliquots of frozen bone marrow. DNA will be extracted and used in genotype analysis. RNA will also be prepared when needed for mutational analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
3P01DK055820-03S1
Application #
6618900
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-05-01
Project End
2003-04-30
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Xu, Huilei; Schaniel, Christoph; Lemischka, Ihor R et al. (2010) Toward a complete in silico, multi-layered embryonic stem cell regulatory network. Wiley Interdiscip Rev Syst Biol Med 2:708-33
Macarthur, Ben D; Ma'ayan, Avi; Lemischka, Ihor R (2009) Systems biology of stem cell fate and cellular reprogramming. Nat Rev Mol Cell Biol 10:672-81
Chang, Kai-Hsin; Nelson, Angelique M; Cao, Hua et al. (2006) Definitive-like erythroid cells derived from human embryonic stem cells coexpress high levels of embryonic and fetal globins with little or no adult globin. Blood 108:1515-23
Duan, Zhijun; Horwitz, Marshall (2005) Gfi-1 takes center stage in hematopoietic stem cells. Trends Mol Med 11:49-52
Richard, Robert E; Siritanaratkul, Noppadol; Jonlin, Erica et al. (2005) Collection of blood stem cells from patients with sickle cell anemia. Blood Cells Mol Dis 35:384-8
Li, Feng-Qian; Person, Richard E; Takemaru, Ken-Ichi et al. (2004) Lymphoid enhancer factor-1 links two hereditary leukemia syndromes through core-binding factor alpha regulation of ELA2. J Biol Chem 279:2873-84
Duan, Zhijun; Li, Feng-Qian; Wechsler, Jeremy et al. (2004) A novel notch protein, N2N, targeted by neutrophil elastase and implicated in hereditary neutropenia. Mol Cell Biol 24:58-70
Doan, Loretta L; Porter, Susan D; Duan, Zhijun et al. (2004) Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells. Nucleic Acids Res 32:2508-19
Escher, Robert; Jones, Antonia; Hagos, Fitsum et al. (2004) Chromosome band 16q22-linked familial AML: exclusion of candidate genes, and possible disease risk modification by NQO1 polymorphisms. Genes Chromosomes Cancer 41:278-82
Zhao, Shengming; Weinreich, Michael A; Ihara, Kenji et al. (2004) In vivo selection of genetically modified erythroid cells using a jak2-based cell growth switch. Mol Ther 10:456-68

Showing the most recent 10 out of 25 publications