Evaluation of energy expenditure has become an indispensable tool for studies involving lean and obese mice. Systems capable of such evaluation are commercially available and can monitor activity, oxygen consumption, CO2 production, feeding and drinking. From this data respiratory quotient, RER and heat produced can be calculated. However the systems are expensive and sophisticated. The Endocrine Division at BIDMC has a sixteen animal monitoring system purchased by outside funds. To make the technology available to PPG investigators, monitoring is offered as a core service. Following monitoring data is provided to the requesting investigator as Excel spreadsheets and graphs plotting the variable of interest in real time. The core assists in experimental design and data analysis. An additional service of the core is assistance with DEXA monitoring for PPG projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
2P01DK056116-06A1
Application #
6928803
Study Section
Special Emphasis Panel (ZDK1-GRB-C (J1))
Project Start
2005-04-01
Project End
2010-03-31
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
6
Fiscal Year
2005
Total Cost
$120,899
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
Sohn, Jong-Woo; Elmquist, Joel K; Williams, Kevin W (2013) Neuronal circuits that regulate feeding behavior and metabolism. Trends Neurosci 36:504-12
Robins, S C; Stewart, I; McNay, D E et al. (2013) ?-Tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors. Nat Commun 4:2049
Kawashima, Junji; Alquier, Thierry; Tsuji, Youki et al. (2012) Ca2+/calmodulin-dependent protein kinase kinase is not involved in hypothalamic AMP-activated protein kinase activation by neuroglucopenia. PLoS One 7:e36335
Dagon, Yossi; Hur, Elizabeth; Zheng, Bin et al. (2012) p70S6 kinase phosphorylates AMPK on serine 491 to mediate leptin's effect on food intake. Cell Metab 16:104-12
McNay, David E G; Briançon, Nadege; Kokoeva, Maia V et al. (2012) Remodeling of the arcuate nucleus energy-balance circuit is inhibited in obese mice. J Clin Invest 122:142-52
Pulinilkunnil, Thomas; He, Huamei; Kong, Dong et al. (2011) Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo. J Biol Chem 286:8798-809
Vella, Kristen R; Ramadoss, Preeti; Lam, Francis S et al. (2011) NPY and MC4R signaling regulate thyroid hormone levels during fasting through both central and peripheral pathways. Cell Metab 14:780-90
Loh, Kim; Fukushima, Atsushi; Zhang, Xinmei et al. (2011) Elevated hypothalamic TCPTP in obesity contributes to cellular leptin resistance. Cell Metab 14:684-99
Chiappini, Franck; Cunha, Lucas L; Harris, Jamie C et al. (2011) Lack of cAMP-response element-binding protein 1 in the hypothalamus causes obesity. J Biol Chem 286:8094-105
Kraus, Daniel; Herman, Mark A; Kahn, Barbara B (2010) Leveraging leptin for type I diabetes? Proc Natl Acad Sci U S A 107:4793-4

Showing the most recent 10 out of 96 publications