Abstract

Since the measurement of peptide hormones and circulating substrates will provide key experimental endpoints for each of the separate projects that make up this program project, funding is requested for an Assay Core laboratory. The primary purpose of this laboratory will be to establish and perform assays for the measurement of the key regulatory peptides necessary for the completion of the three projects. Routine assays for the core will include insulin, leptin, glucose, corticosterone, gastrin releasing peptide, and cholecystokinin. This core will also be responsible for adapting commercially available kits necessary to measure other hormones and peptides, labeling peptides for use as tracers, processing tissues for assay of peptides and development of new techniques and assays. This core will be utilized extensively by all 3 of the individual projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK056863-10
Application #
8073135
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
2012-10-31
Budget Start
2010-05-01
Budget End
2012-10-31
Support Year
10
Fiscal Year
2010
Total Cost
$188,963
Indirect Cost

  Institution

Name
University of Cincinnati
Department
Type
DUNS #
041064767
City
Cincinnati
State
OH
Country
United States
Zip Code
45221

  Publications

Wang, Fei; Yoder, Stephanie M; Yang, Qing et al. (2015) Chronic high-fat feeding increases GIP and GLP-1 secretion without altering body weight. Am J Physiol Gastrointest Liver Physiol 309:G807-15
Ressler, Ilana B; Grayson, Bernadette E; Ulrich-Lai, Yvonne M et al. (2015) Diet-induced obesity exacerbates metabolic and behavioral effects of polycystic ovary syndrome in a rodent model. Am J Physiol Endocrinol Metab 308:E1076-84
Gutierrez-Aguilar, Ruth; Kim, Dong-Hoon; Casimir, Marina et al. (2014) The role of the transcription factor ETV5 in insulin exocytosis. Diabetologia 57:383-91
Grayson, Bernadette E; Hakala-Finch, Andrew P; Kekulawala, Melani et al. (2014) Weight loss by calorie restriction versus bariatric surgery differentially regulates the hypothalamo-pituitary-adrenocortical axis in male rats. Stress 17:484-93
Ressler, Ilana B; Grayson, Bernadette E; Seeley, Randy J (2014) Metabolic, behavioral, and reproductive effects of vertical sleeve gastrectomy in an obese rat model of polycystic ovary syndrome. Obes Surg 24:866-76
Grayson, B E; Fitzgerald, M F; Hakala-Finch, A P et al. (2014) Improvements in hippocampal-dependent memory and microglial infiltration with calorie restriction and gastric bypass surgery, but not with vertical sleeve gastrectomy. Int J Obes (Lond) 38:349-56
Vahl, Torsten P; Aulinger, Benedikt A; Smith, Eric P et al. (2014) Meal feeding improves oral glucose tolerance in male rats and causes adaptations in postprandial islet hormone secretion that are independent of plasma incretins or glycemia. Am J Physiol Endocrinol Metab 307:E784-92
Mul, Joram D; Begg, Denovan P; Haller, April M et al. (2014) MGAT2 deficiency and vertical sleeve gastrectomy have independent metabolic effects in the mouse. Am J Physiol Endocrinol Metab 307:E1065-72
Grayson, Bernadette E; Schneider, Katarina M; Woods, Stephen C et al. (2013) Improved rodent maternal metabolism but reduced intrauterine growth after vertical sleeve gastrectomy. Sci Transl Med 5:199ra112
Mul, Joram D; Begg, Denovan P; Barrera, Jason G et al. (2013) High-fat diet changes the temporal profile of GLP-1 receptor-mediated hypophagia in rats. Am J Physiol Regul Integr Comp Physiol 305:R68-77

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