Abstract

Morphology and Immunohistochemistry Core Scientific Abstract An understanding of which cell types within the retina and kidney are affected by diabetes is essential for effective treatment targeting. The cellular localization of specific proteins and cell death markers, and the quantitative analysis of effects of therapies on retinal and renal structure are integral parts of the projects in the current proposal.
Specific Aim 1 : The purpose of the Morphology and Immunohistochemistry Core is to aid in the execution of anatomically- based experiments by providing: a. technical support for tissue collection and processing, b. expert assistance in evaluation of retinal and renal histopathology, and c. quantify reagents and technical support for immunohistochemistry.
Specific Aim 2 : The Morphology and Immunohistochemistry Core will facilitate and stimulate research in individual projects by providing expert assistance and special facilities essential for the projects but beyond the current capabilities of investigators. It will also provide quality control and facilitate interaction between investigators, and provide teaching in these technical areas.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK057733-03
Application #
6659262
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
$94,628
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Alexander, Nathan S; Palczewska, Grazyna; Stremplewski, Patrycjusz et al. (2016) Image registration and averaging of low laser power two-photon fluorescence images of mouse retina. Biomed Opt Express 7:2671-91
Kern, Elizabeth F O; Erhard, Penny; Sun, Wanjie et al. (2010) Early urinary markers of diabetic kidney disease: a nested case-control study from the Diabetes Control and Complications Trial (DCCT). Am J Kidney Dis 55:824-34
Kern, Timothy S; Du, Yunpeng; Miller, Casey M et al. (2010) Overexpression of Bcl-2 in vascular endothelium inhibits the microvascular lesions of diabetic retinopathy. Am J Pathol 176:2550-8
Ozdemir, Aylin M; Hopfer, Ulrich; Rosca, Mariana V et al. (2008) Effects of advanced glycation end product modification on proximal tubule epithelial cell processing of albumin. Am J Nephrol 28:14-24
Kern, Timothy S (2007) Contributions of inflammatory processes to the development of the early stages of diabetic retinopathy. Exp Diabetes Res 2007:95103
Lu, Liang; Erhard, Penny; Salomon, Robert G et al. (2007) Serum vitamin E and oxidative protein modification in hemodialysis: a randomized clinical trial. Am J Kidney Dis 50:305-13
Kern, Timothy S; Miller, Casey M; Du, Yunpeng et al. (2007) Topical administration of nepafenac inhibits diabetes-induced retinal microvascular disease and underlying abnormalities of retinal metabolism and physiology. Diabetes 56:373-9
Staniszewska, Magdalena M; Nagaraj, Ram H (2006) Upregulation of glyoxalase I fails to normalize methylglyoxal levels: a possible mechanism for biochemical changes in diabetic mouse lenses. Mol Cell Biochem 288:29-36
Genuth, Saul; Sun, Wanjie; Cleary, Patricia et al. (2005) Glycation and carboxymethyllysine levels in skin collagen predict the risk of future 10-year progression of diabetic retinopathy and nephropathy in the diabetes control and complications trial and epidemiology of diabetes interventions and complications p Diabetes 54:3103-11
Sell, David R; Biemel, Klaus M; Reihl, Oliver et al. (2005) Glucosepane is a major protein cross-link of the senescent human extracellular matrix. Relationship with diabetes. J Biol Chem 280:12310-5

Showing the most recent 10 out of 32 publications