The Animal/Morphology Core B will provide SAMP1/Yit mice for use in each of the individual projects. Maintaining the SAMP1/Yit breeding colony and experimental animals in a common centralized facility under uniform environmental conditions is crucial to the success of the program because of evidence that environmental cofactors such as colonization of the gut with bacteria flora can effect the presence or course of disease. Centralization of animal production will also reduce costs by eliminating redundant breeding colonies, and ultimately reduce the numbers of animals required by ensuring efficient utilization by each of the projects.
A second aim i s to provide centralized histological services and assistance with immunohistochemistry, digital fluorescence and brightfield microscopy for each of the projects.
The third aim of the Animal /Morphology core is to provide centralized histopathological analyses of inflammation and other morphological parameters to investigators within the program. A centralized core component for analysis of the histopathological features of ileitis in this model will provide a common framework for interpretation of data generated by each of the four projects. A strength of this component is the availability of two experienced GI pathologists, Dr. Moskaluk and Dr. Nast, to perform histopathological scoring of samples to each of the projects and to provide ongoing consultative service to the projects concerning the pathological features of disease in this model.
The fourth aim i s to provide a resource for providing mice with induced mutations on induced mutations into an appropriate background can be accomplished in 3 to 4 generations. Dr. Cohn will direct the core and Dr. Moskaluk will serve as co-director. A committee consisting of the core director, the co-director, and Dr. Cominelli, the PI of the program will address routine quality control and prioritization issues. An oversight committee will review the operation of the core on yearly basis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK057880-03
Application #
6651779
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
$107,756
Indirect Cost
Name
University of Virginia
Department
Type
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Ernst, P B; Erickson, L D; Loo, W M et al. (2012) Spontaneous autoimmune gastritis and hypochlorhydria are manifest in the ileitis-prone SAMP1/YitFcs mice. Am J Physiol Gastrointest Liver Physiol 302:G105-15
Lou, Yuefen; Lu, Xiaojiong; Dang, Xitong (2012) FOXO1 Up-Regulates Human L-selectin Expression Through Binding to a Consensus FOXO1 Motif. Gene Regul Syst Bio 6:139-49
Reuter, Brian K; Pastorelli, Luca; Brogi, Marco et al. (2011) Spontaneous, immune-mediated gastric inflammation in SAMP1/YitFc mice, a model of Crohn's-like gastritis. Gastroenterology 141:1709-19
Pizarro, Theresa T; Pastorelli, Luca; Bamias, Giorgos et al. (2011) SAMP1/YitFc mouse strain: a spontaneous model of Crohn's disease-like ileitis. Inflamm Bowel Dis 17:2566-84
Gorfu, Gezahegn; Rivera-Nieves, Jesus; Hoang, Sharon et al. (2010) Beta7 integrin deficiency suppresses B cell homing and attenuates chronic ileitis in SAMP1/YitFc mice. J Immunol 185:5561-8
Shanahan, Michael T; Vidrich, Alda; Shirafuji, Yoshinori et al. (2010) Elevated expression of Paneth cell CRS4C in ileitis-prone SAMP1/YitFc mice: regional distribution, subcellular localization, and mechanism of action. J Biol Chem 285:7493-504
Pastorelli, Luca; Garg, Rekha R; Hoang, Sharon B et al. (2010) Epithelial-derived IL-33 and its receptor ST2 are dysregulated in ulcerative colitis and in experimental Th1/Th2 driven enteritis. Proc Natl Acad Sci U S A 107:8017-22
Reuter, Brian K; Pizarro, Theresa T (2009) Mechanisms of tight junction dysregulation in the SAMP1/YitFc model of Crohn's disease-like ileitis. Ann N Y Acad Sci 1165:301-7
Dang, Xitong; Raffler, Nikolai A; Ley, Klaus (2009) Transcriptional regulation of mouse L-selectin. Biochim Biophys Acta 1789:146-52
Gorfu, G; Rivera-Nieves, J; Ley, K (2009) Role of beta7 integrins in intestinal lymphocyte homing and retention. Curr Mol Med 9:836-50

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