Worm microinjection is technically demanding and utilizes expensive and sensitive instrumentation. These requirements necessitate operation of a shared microinjection facility to minimize equipment costs and project startup times, and to maximize laboratory efficiency. The proposed Worm Core will provide microinjection services, microinjection training, and maintenance of a shared microinjection system. To minimize costs and space utilization, and to focus the laboratories on experimental aspects of their proposed projects, the Work Core will provide centralized support for worm husbandry including production of work culture material and preparation of large scale work cultures. In addition, the Work Core will maintain a catalogued collection of plasmids and other specialized molecular reagents required for C. elegans work, and a catalogued backup archiving system for all previously and newly created worm strains.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK058212-03
Application #
6618905
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
$159,857
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Benninger, Richard K P; Piston, David W (2014) Cellular communication and heterogeneity in pancreatic islet insulin secretion dynamics. Trends Endocrinol Metab 25:399-406
Kumar, Ankur N; Short, Kurt W; Piston, David W (2013) A motion correction framework for time series sequences in microscopy images. Microsc Microanal 19:433-50
Ustione, Alessandro; Piston, David W (2012) Dopamine synthesis and D3 receptor activation in pancreatic ?-cells regulates insulin secretion and intracellular [Ca(2+)] oscillations. Mol Endocrinol 26:1928-40
Meissner, Barbara; Warner, Adam; Wong, Kim et al. (2009) An integrated strategy to study muscle development and myofilament structure in Caenorhabditis elegans. PLoS Genet 5:e1000537
Watson, Joseph D; Wang, Shenglong; Von Stetina, Stephen E et al. (2008) Complementary RNA amplification methods enhance microarray identification of transcripts expressed in the C. elegans nervous system. BMC Genomics 9:84
Fox, Rebecca M; Watson, Joseph D; Von Stetina, Stephen E et al. (2007) The embryonic muscle transcriptome of Caenorhabditis elegans. Genome Biol 8:R188
Von Stetina, Stephen E; Watson, Joseph D; Fox, Rebecca M et al. (2007) Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system. Genome Biol 8:R135
Von Stetina, Stephen E; Fox, Rebecca M; Watkins, Kathie L et al. (2007) UNC-4 represses CEH-12/HB9 to specify synaptic inputs to VA motor neurons in C. elegans. Genes Dev 21:332-46
Denton, Jerod; Nehrke, Keith; Yin, Xiaoyan et al. (2006) Altered gating and regulation of a carboxy-terminal ClC channel mutant expressed in the Caenorhabditis elegans oocyte. Am J Physiol Cell Physiol 290:C1109-18
Touroutine, Denis; Fox, Rebecca M; Von Stetina, Stephen E et al. (2005) acr-16 encodes an essential subunit of the levamisole-resistant nicotinic receptor at the Caenorhabditis elegans neuromuscular junction. J Biol Chem 280:27013-21

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