Abstract

application) The basic study model for this program is primary cultured hepatocytes. The role of the Cell Isolation/Transgenic Core is to provide freshly isolated rat and mouse hepatocytes and mitochondria to program investigators. The Core will: 1. isolate and distribute hepatocytes and mitochondria from Sprague Dawley rats. 2. Isolate and distribute hepatocytes from various transgenic mice and the corresponding wild type mice. 3. House and maintain rats and transgenic mice, including knockout mice for iNOS, GD3 synthase, Bid and Bax, transgenic mice overexpressing Bcl-2 and Bcl-x, and corresponding wild-type strains. Core personnel are well trained and experienced in isolating and culturing primary hepatocytes and preparing mitochondria by homogenization and differential centrifugation. Core staff will also provide consultation on these preparations for all project investigators to meet their specific needs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK059340-02
Application #
6655818
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2002-06-01
Project End
2003-05-31
Budget Start
Budget End
Support Year
2
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Dolganiuc, Angela; Thomes, Paul G; Ding, Wen-Xing et al. (2012) Autophagy in alcohol-induced liver diseases. Alcohol Clin Exp Res 36:1301-8
Kim, Insil; Lemasters, John J (2011) Mitochondrial degradation by autophagy (mitophagy) in GFP-LC3 transgenic hepatocytes during nutrient deprivation. Am J Physiol Cell Physiol 300:C308-17
Kim, Insil; Lemasters, John J (2011) Mitophagy selectively degrades individual damaged mitochondria after photoirradiation. Antioxid Redox Signal 14:1919-28
Rodriguez-Enriquez, Sara; Kai, Yoichiro; Maldonado, Eduardo et al. (2009) Roles of mitophagy and the mitochondrial permeability transition in remodeling of cultured rat hepatocytes. Autophagy 5:1099-106
Kim, Insil; Rodriguez-Enriquez, Sara; Lemasters, John J (2007) Selective degradation of mitochondria by mitophagy. Arch Biochem Biophys 462:245-53
Adachi, Masayuki; Osawa, Yosuke; Uchinami, Hiroshi et al. (2007) The forkhead transcription factor FoxO1 regulates proliferation and transdifferentiation of hepatic stellate cells. Gastroenterology 132:1434-46
Lemasters, John J (2007) Modulation of mitochondrial membrane permeability in pathogenesis, autophagy and control of metabolism. J Gastroenterol Hepatol 22 Suppl 1:S31-7
Hammond, Linda E; Albright, Craig D; He, Lihua et al. (2007) Increased oxidative stress is associated with balanced increases in hepatocyte apoptosis and proliferation in glycerol-3-phosphate acyltransferase-1 deficient mice. Exp Mol Pathol 82:210-9
Kim, Jae-Sung; Lemasters, John J (2006) Opioid receptor-independent protection of ischemic rat hepatocytes by morphine. Biochem Biophys Res Commun 351:958-64
Kim, Jae-Sung; Jin, Yingai; Lemasters, John J (2006) Reactive oxygen species, but not Ca2+ overloading, trigger pH- and mitochondrial permeability transition-dependent death of adult rat myocytes after ischemia-reperfusion. Am J Physiol Heart Circ Physiol 290:H2024-34

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