The Imaging Core will provide the microscopy and imaging instruments and expertise to the individual Projects of Program Project Grant. The facilities and personnel of the Imaging Core are drawn from the Biomedical Imaging Group (BIG) at UMMS, a long established and productive group of researchers who have made significant contributions to cell biology through advances in high spatial and temporal resolution fluorescence microscopy, and the development of hardware, software, algorithms and techniques. The Imaging Core is located within the laboratory of the Biomedical Imaging Group, which is on the same floor as the laboratories of the other P.l.s of the Program Project. The central instruments provided are: 1) A Digital Imaging Microscope for multi-color, high-spatial resolution 3-D deconvolution imaging of fixed and living cells;2) A unique, custom-built, multi-color, high-temporal resolution micrscope for fast 2-D, and 3-D deconvolution imaging of GFP (and variants) and otherfluorophores in living cells overtime;3) A unique multi-color Total Internal Reflection Fluorescence Microscope (TIRFM) which can be combined with fast 3-D imaging, providing high-resolution images of the spatial domain close (<300 nm, mean ~100 nm) to the plasma membrane in Iving cells;4) custom deconvolution software developed by BIG and a Beowulf computing cluster to rapidly reconstuct 5-D (multi-color 3-D time series) images of living cells;5) state-of-art color graphics workstations equipped with custom visualization and analysis software for 5-D image analysis; 6) Image archivial storage and retrieval file servers for safe on-line storage of the large (terabytes) amount of data generated by the Cores instruments and analysis programs. In particluar, the Core will provide continuing research and development of the imaging and analysis with TIRF microscopy as it is critical to the specific aims of Projects 1, 2 and 3. The five faculty members of the Core (1.1 FTE) will provide the Program Project investigators with expertise in: the design of imaging experiments including modifications to equipment, simulation, and testing of experimental protocols;design, modification and application of image processing algorithms and software for image analysis (much analysis of data is carried out by the core), and maintanence or modification of all neccesary Core resources, including opto-electronics, computers and software systems.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK060564-09
Application #
8068303
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2010-05-01
Budget End
2011-04-30
Support Year
9
Fiscal Year
2010
Total Cost
$198,824
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Type
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655
Ly, Socheata; Navaroli, Deanna M; Didiot, Marie-C├ęcile et al. (2017) Visualization of self-delivering hydrophobically modified siRNA cellular internalization. Nucleic Acids Res 45:15-25
Rohatgi, R A; Janusis, J; Leonard, D et al. (2015) Beclin 1 regulates growth factor receptor signaling in breast cancer. Oncogene 34:5352-62
Stockler, Sylvia; Corvera, Silvia; Lambright, David et al. (2014) Single point mutation in Rabenosyn-5 in a female with intractable seizures and evidence of defective endocytotic trafficking. Orphanet J Rare Dis 9:141
Malaby, Andrew W; van den Berg, Bert; Lambright, David G (2013) Structural basis for membrane recruitment and allosteric activation of cytohesin family Arf GTPase exchange factors. Proc Natl Acad Sci U S A 110:14213-8
Davey, Jonathan R; Humphrey, Sean J; Junutula, Jagath R et al. (2012) TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes. Traffic 13:1429-41
Li, Jian; Malaby, Andrew W; Famulok, Michael et al. (2012) Grp1 plays a key role in linking insulin signaling to glut4 recycling. Dev Cell 22:1286-98
Young, James L; Mora, Alfonso; Cerny, Anna et al. (2012) CD14 deficiency impacts glucose homeostasis in mice through altered adrenal tone. PLoS One 7:e29688
Navaroli, Deanna M; Bellve, Karl D; Standley, Clive et al. (2012) Rabenosyn-5 defines the fate of the transferrin receptor following clathrin-mediated endocytosis. Proc Natl Acad Sci U S A 109:E471-80
Tan, Shi-Xiong; Ng, Yvonne; Burchfield, James G et al. (2012) The Rab GTPase-activating protein TBC1D4/AS160 contains an atypical phosphotyrosine-binding domain that interacts with plasma membrane phospholipids to facilitate GLUT4 trafficking in adipocytes. Mol Cell Biol 32:4946-59
Xie, Xiangyang; Gong, Zhenwei; Mansuy-Aubert, Virginie et al. (2011) C2 domain-containing phosphoprotein CDP138 regulates GLUT4 insertion into the plasma membrane. Cell Metab 14:378-89

Showing the most recent 10 out of 33 publications