The services in the Mouse Metabolic Phenotyping Core (Core C) draw upon the expertise of many investigators at UTSW to provide comprehensive analytical capabilities that are on par with the best facilities in the world. We are looking back on 5 successful years during which we have expanded our assay capabilities and supported Projects 1 through 3 during the ongoing funding period with our comprehensive phenotyping panel under the direction of Dr. Jay Horton. We have made major steps towards establishing a state-of-the-art sphingolipidomics suite beyond the originally described standard phenotyping panel. The availability of standardized assays speeds the pace of our research, facilitates inter-lab comparisons of data, and provides a common platform for the necessary close interactions between investigators. Animal models that allow careful characterization of the metabolic effects of obesity and that have an altered communication axis between the three critical tissues studied here are vital to understand the pathogenesis of disease. Given the number of phenotypic measurements, the range of target metabolites, and the number of different animal models investigated, it is necessary to provide uniform measurements and to have quality control guidelines in place. To facilitate the research for Projects 1-3, Core C will provide comprehensive, state-of-the-art measurements of multiple parameters, with measurements involving mass spectrometry and physiological read outs that include lipid, glucose, and metabokine assays. The technical expertise Core C offers will bring multiple benefits to project investigators, including the following: ? Centralized Core services are more cost effective and prevent the duplication of reagents across PPG investigators. ? Provides consistency in various assays across laboratories. ? Provides consistent quality control measures. ? Makes available technically challenging assays, such as hyperinsulinemic-euglycemic clamps, that cannot be established in individual laboratories. ? Frees investigators from routine work to focus on the intellectual challenges of the projects.

Public Health Relevance

Core C, the Mouse Metabolic Phenotyping Core, provides comprehensive analytical capabilities for the phenotyping of intact mice and the analysis of plasma and tissue samples. Given the number of phenotypic measurements, the range of target metabolites, and the number of different animal models investigated, it is necessary to provide uniform measurements and to have quality control guidelines in place. To facilitate the research for Projects 1-3, Core C will provide comprehensive, state-of-the-art measurements of multiple parameters, with measurements involving mass spectrometry and physiological read outs that include lipid, glucose and metabokine assays. This will free investigators from routine work to focus on the intellectual challenges of the projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
2P01DK088761-06
Application #
8933739
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2015-07-01
Budget End
2016-06-30
Support Year
6
Fiscal Year
2015
Total Cost
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Type
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
Jia, Lin; Chang, Xiuli; Qian, Shuwen et al. (2018) Hepatocyte toll-like receptor 4 deficiency protects against alcohol-induced fatty liver disease. Mol Metab 14:121-129
Wang, Qiong A; Zhang, Fang; Jiang, Lei et al. (2018) PPAR? and its Role in Adipocyte Homeostasis and Thiazolidinedione-Mediated Insulin Sensitization. Mol Cell Biol :
Crewe, Clair; Joffin, Nolwenn; Rutkowski, Joseph M et al. (2018) An Endothelial-to-Adipocyte Extracellular Vesicle Axis Governed by Metabolic State. Cell 175:695-708.e13
Scherer, Philipp E (2018) The many secret lives of adipocytes: implications for diabetes. Diabetologia :
Zhang, Fang; Hao, Guiyang; Shao, Mengle et al. (2018) An Adipose Tissue Atlas: An Image-Guided Identification of Human-like BAT and Beige Depots in Rodents. Cell Metab 27:252-262.e3
Chen, Xi; Ayala, Iriscilla; Shannon, Chris et al. (2018) The Diabetes Gene and Wnt Pathway Effector TCF7L2 Regulates Adipocyte Development and Function. Diabetes 67:554-568
Kruglikov, Ilja L; Zhang, Zhuzhen; Scherer, Philipp E (2018) The Role of Immature and Mature Adipocytes in Hair Cycling. Trends Endocrinol Metab :
Xia, Jonathan Y; Sun, Kai; Hepler, Chelsea et al. (2018) Acute loss of adipose tissue-derived adiponectin triggers immediate metabolic deterioration in mice. Diabetologia 61:932-941
Kusminski, Christine M; Scherer, Philipp E (2018) New zoning laws enforced by glucagon. Proc Natl Acad Sci U S A 115:4308-4310
Ye, Risheng; Gordillo, Ruth; Shao, Mengle et al. (2018) Intracellular lipid metabolism impairs ? cell compensation during diet-induced obesity. J Clin Invest 128:1178-1189

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