Abstract

The overall objective of the Core is to provide resources and expertise to perform and analyze data from gene expression, miRNA expression, ChIP-seq, and microbiota profiling assays that identify disease-specific transcriptional patterns and shifts in microbiota abundance in tissue of patients with IBD. Additionally, the Core will enable project investigators to perform functional validation in patient- derived organoid experimental systems to substantiate and guide their hypotheses obtained in patient samples and animal models. This will be facilitated through access to human samples from well- characterized adult and pediatric IBD patients and controls.

Public Health Relevance

The inflammatory bowel diseases (IBD), primarily Crohn?s disease and ulcerative colitis, are highly heterogeneous in their presentation, progression, and response to treatment. The Human Tissue and Genomics Core aims to provide valuable tissue material, clinical history, and access to cutting edge genomics technologies in order to facilitate the discovery of biomarkers and biological mechanisms of distinct disease phenotypes within IBD.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK094779-07
Application #
10018858
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2020-07-01
Budget End
2021-06-30
Support Year
7
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Cronan, Mark R; Matty, Molly A; Rosenberg, Allison F et al. (2018) An explant technique for high-resolution imaging and manipulation of mycobacterial granulomas. Nat Methods 15:1098-1107
Ellermann, Melissa; Sartor, R Balfour (2018) Intestinal bacterial biofilms modulate mucosal immune responses. J Immunol Sci 2:13-18
Pushalkar, Smruti; Hundeyin, Mautin; Daley, Donnele et al. (2018) The Pancreatic Cancer Microbiome Promotes Oncogenesis by Induction of Innate and Adaptive Immune Suppression. Cancer Discov 8:403-416
Truax, Agnieszka D; Chen, Liang; Tam, Jason W et al. (2018) The Inhibitory Innate Immune Sensor NLRP12 Maintains a Threshold against Obesity by Regulating Gut Microbiota Homeostasis. Cell Host Microbe 24:364-378.e6
Vázquez-Baeza, Yoshiki; Gonzalez, Antonio; Xu, Zhenjiang Zech et al. (2018) Guiding longitudinal sampling in IBD cohorts. Gut 67:1743-1745
Ho, G-T; Aird, R E; Liu, B et al. (2018) MDR1 deficiency impairs mitochondrial homeostasis and promotes intestinal inflammation. Mucosal Immunol 11:120-130
Keith, Benjamin P; Barrow, Jasmine B; Toyonaga, Takahiko et al. (2018) Colonic epithelial miR-31 associates with the development of Crohn's phenotypes. JCI Insight 3:
Huang, Juin-Hua; Liu, Chu-Yu; Wu, Sheng-Yang et al. (2018) NLRX1 Facilitates Histoplasma capsulatum-Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages. Front Immunol 9:2761
Weiser, Matthew; Simon, Jeremy M; Kochar, Bharati et al. (2018) Molecular classification of Crohn's disease reveals two clinically relevant subtypes. Gut 67:36-42
Sartor, R Balfour; Wu, Gary D (2017) Roles for Intestinal Bacteria, Viruses, and Fungi in Pathogenesis of Inflammatory Bowel Diseases and Therapeutic Approaches. Gastroenterology 152:327-339.e4

Showing the most recent 10 out of 35 publications